Accumulation and intracellular compartmentation of lithium ions in Saccharomyces cerevisiae

Joy Perkins; Geoffrey M. Gadd

doi:10.1111/j.1574-6968.1993.tb06039.x

Accumulation and intracellular compartmentation of lithium ions in Saccharomyces cerevisiae

Joy Perkins, Geoffrey M. Gadd (Lead / Corresponding author)

University of Dundee

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

Accumulation of Li⁺ in Saccharomyces cerevisiae X2180-1B occured via an apparent stoichiometric relationship of 1 : 1 (K⁺/Li⁺) when S. cerevisiae was incubated in the presence of 5 and 10 mM LiCl for 3 h. Other cellular cations (Mg²⁺, Ca²⁺ and Na⁺) did not vary on Li⁺ accumulation, although lithium chemistry dictates a degree of similarity to Group I and II metal cations. Compartmentation of Li⁺ was mainly in the vacuole which accounted for 85% of the Li⁺ accumulated after a 6-h incubation period. The remainder was located in the cytosol with negligible amounts being bound to cell fragments including the cell wall. Transmission electron microscopy of Li⁺-loaded cells revealed enlarged vacuoles compared with control cells. This asymmetric cellular distribution may therefore enhance tolerance of S. cerevisiae to Li⁺ and ensure that essential metabolic processes in the cytosol are not disrupted.

Original language	English
Pages (from-to)	255-260
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	107
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb06039.x
Publication status	Published - 1 Mar 1993

Keywords

Intracellular compartmentation
Lithium
Potassium
Saccharomyces cerevisiae
Vacuole

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1993.tb06039.x

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title = "Accumulation and intracellular compartmentation of lithium ions in Saccharomyces cerevisiae",

abstract = "Accumulation of Li+ in Saccharomyces cerevisiae X2180-1B occured via an apparent stoichiometric relationship of 1 : 1 (K+/Li+) when S. cerevisiae was incubated in the presence of 5 and 10 mM LiCl for 3 h. Other cellular cations (Mg2+, Ca2+ and Na+) did not vary on Li+ accumulation, although lithium chemistry dictates a degree of similarity to Group I and II metal cations. Compartmentation of Li+ was mainly in the vacuole which accounted for 85% of the Li+ accumulated after a 6-h incubation period. The remainder was located in the cytosol with negligible amounts being bound to cell fragments including the cell wall. Transmission electron microscopy of Li+-loaded cells revealed enlarged vacuoles compared with control cells. This asymmetric cellular distribution may therefore enhance tolerance of S. cerevisiae to Li+ and ensure that essential metabolic processes in the cytosol are not disrupted.",

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AU - Gadd, Geoffrey M.

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Accumulation and intracellular compartmentation of lithium ions in Saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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Erratum: Accumulation and intracellular compartmentation of lithium ions in Saccharomyces cerevisiae (FEMS Microbiology Letters, pp. 255-260)

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