Activation of a novel non-selective cation channel by alloxan and H2O2 in the rat insulin-secreting cell line CRI-G1

P. S. Herson, M. L. Ashford

    Research output: Contribution to journalArticle

    58 Citations (Scopus)

    Abstract

    1. Alloxan and its auto-oxidation product hydrogen peroxide (H2O2) irreversibly depolarize insulinoma cells by opening a non-selective cation channel. The channel opened is characterized by a linear current-voltage relation with a conductance of approximately 70 pS and very slow kinetics (of the order of seconds). 2. Cells are protected against the alloxan-induced channel opening and consequent cell depolarization by the presence of H2O2 and hydroxyl radical scavengers. 3. The free radical-activated non-selective cation channel is not operative in isolated patches but can be activated by the application of beta-NAD+ to the cytoplasmic aspect of the membrane.
    Original languageEnglish
    Pages (from-to)59-66
    Number of pages8
    JournalJournal of Physiology
    Volume501
    Issue number( Pt 1)
    Publication statusPublished - 15 May 1997

    Keywords

    • Animals
    • Niacinamide
    • Thiourea
    • Hydrogen Peroxide
    • Insulinoma
    • Islets of Langerhans
    • Reactive Oxygen Species
    • Rats
    • Patch-Clamp Techniques
    • Tumor Cells, Cultured
    • Cell Polarity
    • Cysteine
    • Alloxan
    • Free Radical Scavengers
    • Membrane Potentials

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