TY - JOUR
T1 - Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains
AU - Monostori, Eva
AU - Desai, Dev
AU - Brown, Marion H.
AU - Cantrell, Doreen A.
AU - Crumpton, Michael J.
PY - 1990/2/1
Y1 - 1990/2/1
N2 - The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T112 and T113, PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 γ-chain. The combination of T112 and T113 mAb also induced phosphorylation of the TCR ζ-chain. The phosphorylated ζ-polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T112 and T113 mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 γ-chain and the tyrosine phosphorylation of the ζ-chain, respectively.
AB - The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T112 and T113, PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 γ-chain. The combination of T112 and T113 mAb also induced phosphorylation of the TCR ζ-chain. The phosphorylated ζ-polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T112 and T113 mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 γ-chain and the tyrosine phosphorylation of the ζ-chain, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0025125721&partnerID=8YFLogxK
M3 - Article
C2 - 1688586
AN - SCOPUS:0025125721
SN - 0022-1767
VL - 144
SP - 1010
EP - 1014
JO - Journal of Immunology
JF - Journal of Immunology
IS - 3
ER -