Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains

Eva Monostori; Dev Desai; Marion H. Brown; Doreen A. Cantrell; Michael J. Crumpton

Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains

Eva Monostori
, Dev Desai
, Marion H. Brown
, Doreen A. Cantrell
, Michael J. Crumpton

Cell Signalling and Immunology

Research output: Contribution to journal › Article › peer-review

86 Citations (Scopus)

Abstract

The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T11₂ and T11₃, PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 γ-chain. The combination of T11₂ and T11₃ mAb also induced phosphorylation of the TCR ζ-chain. The phosphorylated ζ-polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T11₂ and T11₃ mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 γ-chain and the tyrosine phosphorylation of the ζ-chain, respectively.

Original language	English
Pages (from-to)	1010-1014
Number of pages	5
Journal	Journal of Immunology
Volume	144
Issue number	3
Publication status	Published - 1 Feb 1990

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Cite this

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title = "Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains",

abstract = "The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T112 and T113, PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 γ-chain. The combination of T112 and T113 mAb also induced phosphorylation of the TCR ζ-chain. The phosphorylated ζ-polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T112 and T113 mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 γ-chain and the tyrosine phosphorylation of the ζ-chain, respectively.",

author = "Eva Monostori and Dev Desai and Brown, \{Marion H.\} and Cantrell, \{Doreen A.\} and Crumpton, \{Michael J.\}",

year = "1990",

month = feb,

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language = "English",

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pages = "1010--1014",

journal = "Journal of Immunology",

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TY - JOUR

T1 - Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains

AU - Monostori, Eva

AU - Desai, Dev

AU - Brown, Marion H.

AU - Cantrell, Doreen A.

AU - Crumpton, Michael J.

PY - 1990/2/1

Y1 - 1990/2/1

N2 - The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T112 and T113, PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 γ-chain. The combination of T112 and T113 mAb also induced phosphorylation of the TCR ζ-chain. The phosphorylated ζ-polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T112 and T113 mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 γ-chain and the tyrosine phosphorylation of the ζ-chain, respectively.

AB - The phosphorylation of the invariant chains associated with the human TCR has been investigated after the stimulation of T lymphocytes with CD2 mAb T112 and T113, PHA, or phorbol 12,13-dibutyrate. As described previously, stimulation of T cells with either CD2 mAb or phorbol 12,13-dibutyrate resulted in the phosphorylation of the CD3 γ-chain. The combination of T112 and T113 mAb also induced phosphorylation of the TCR ζ-chain. The phosphorylated ζ-polypeptide of CD2-activated cells was immunoprecipitated with antiphosphotyrosine antibodies and migrated to a 21- to 23-kDa position during SDS/PAGE. These results indicate that stimulation of human T cells via the CD2 Ag with the T112 and T113 mAb activates not only protein kinase C but also tyrosine kinase(s), resulting in the phosphorylation of the CD3 γ-chain and the tyrosine phosphorylation of the ζ-chain, respectively.

UR - https://www.scopus.com/pages/publications/0025125721

M3 - Article

C2 - 1688586

AN - SCOPUS:0025125721

SN - 0022-1767

VL - 144

SP - 1010

EP - 1014

JO - Journal of Immunology

JF - Journal of Immunology

IS - 3

ER -

Activation of human T lymphocytes via the CD2 antigen results in tyrosine phosphorylation of T cell antigen receptor ζ-chains

Abstract

ASJC Scopus subject areas

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