Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells

Yair N. Doza; Ana Cuenda; Gareth M. Thomas; Philip Cohen; Angel R. Nebreda

doi:10.1016/0014-5793(95)00346-B

Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells

Yair N. Doza, Ana Cuenda, Gareth M. Thomas, Philip Cohen, Angel R. Nebreda

Research output: Contribution to journal › Article › peer-review

78 Citations (Scopus)

Abstract

A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme.

Original language	English
Pages (from-to)	223-228
Number of pages	6
Journal	FEBS Letters
Volume	364
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(95)00346-B
Publication status	Published - 8 May 1995

Keywords

Arsenite
MAP kinase
Phosphotyrosine
Protein kinase
Stress

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)00346-B

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abstract = "A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme.",

keywords = "Arsenite, MAP kinase, Phosphotyrosine, Protein kinase, Stress",

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T1 - Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells

AU - Doza, Yair N.

AU - Cuenda, Ana

AU - Thomas, Gareth M.

AU - Cohen, Philip

AU - Nebreda, Angel R.

PY - 1995/5/8

Y1 - 1995/5/8

N2 - A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme.

AB - A MAP kinase homologue, termed the reactivating kinase (RK), lies in a signalling pathway which mediates cellular responses to stress. Here we demonstrate that the stress-induced activation of the RK in human KB cells is accompanied by the phosphorylation of Thr-180 and Tyr-182, and that the phosphorylation of both residues is required for the activation of this enzyme.

KW - Arsenite

KW - MAP kinase

KW - Phosphotyrosine

KW - Protein kinase

KW - Stress

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U2 - 10.1016/0014-5793(95)00346-B

DO - 10.1016/0014-5793(95)00346-B

M3 - Article

C2 - 7750576

AN - SCOPUS:0028969480

SN - 0014-5793

VL - 364

SP - 223

EP - 228

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Activation of the MAP kinase homologue RK requires the phosphorylation of Thr-180 and Tyr-182 and both residues are phosphorylated in chemically stressed KB cells

Abstract

Keywords

ASJC Scopus subject areas

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