Activation of the MAP kinase pathway by the protein kinase raf

Louise R. Howe; Sally J. Leevers; Néstor Gómez; Sara Nakielny; Philip Cohen; Christopher J. Marshall

doi:10.1016/0092-8674(92)90361-F

Activation of the MAP kinase pathway by the protein kinase raf

Louise R. Howe, Sally J. Leevers, Néstor Gómez, Sara Nakielny, Philip Cohen, Christopher J. Marshall

Research output: Contribution to journal › Article › peer-review

773 Citations (Scopus)

Abstract

Both MAP kinases and the protein kinase p74^raf-1 are activated by many growth factors in a c-ras-dependent manner and by oncogenic p21^ras. We were therefore interested in determining the relationship between MAP kinases and raf. The MAP kinase ERK2 is activated by expression of oncogenically activated raf, independently of cellular ras. Overexpressed p74^raf-1 potentiates activation of ERK2 by EGF and TPA. MAP kinase kinase inactivated by phosphatase 2A treatment is phosphorylated and reactivated by incubation with p74^raf-1 immunoprecipitated from phorbol estertreated cells. We conclude that raf protein kinase is upstream of MAP kinases and is either a MAP kinase kinase kinase or a MAP kinase kinase kinase kinase.

Original language	English
Pages (from-to)	335-342
Number of pages	8
Journal	Cell
Volume	71
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(92)90361-F
Publication status	Published - 16 Oct 1992

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology

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title = "Activation of the MAP kinase pathway by the protein kinase raf",

abstract = "Both MAP kinases and the protein kinase p74raf-1 are activated by many growth factors in a c-ras-dependent manner and by oncogenic p21ras. We were therefore interested in determining the relationship between MAP kinases and raf. The MAP kinase ERK2 is activated by expression of oncogenically activated raf, independently of cellular ras. Overexpressed p74raf-1 potentiates activation of ERK2 by EGF and TPA. MAP kinase kinase inactivated by phosphatase 2A treatment is phosphorylated and reactivated by incubation with p74raf-1 immunoprecipitated from phorbol estertreated cells. We conclude that raf protein kinase is upstream of MAP kinases and is either a MAP kinase kinase kinase or a MAP kinase kinase kinase kinase.",

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T1 - Activation of the MAP kinase pathway by the protein kinase raf

AU - Howe, Louise R.

AU - Leevers, Sally J.

AU - Gómez, Néstor

AU - Nakielny, Sara

AU - Cohen, Philip

AU - Marshall, Christopher J.

PY - 1992/10/16

Y1 - 1992/10/16

N2 - Both MAP kinases and the protein kinase p74raf-1 are activated by many growth factors in a c-ras-dependent manner and by oncogenic p21ras. We were therefore interested in determining the relationship between MAP kinases and raf. The MAP kinase ERK2 is activated by expression of oncogenically activated raf, independently of cellular ras. Overexpressed p74raf-1 potentiates activation of ERK2 by EGF and TPA. MAP kinase kinase inactivated by phosphatase 2A treatment is phosphorylated and reactivated by incubation with p74raf-1 immunoprecipitated from phorbol estertreated cells. We conclude that raf protein kinase is upstream of MAP kinases and is either a MAP kinase kinase kinase or a MAP kinase kinase kinase kinase.

AB - Both MAP kinases and the protein kinase p74raf-1 are activated by many growth factors in a c-ras-dependent manner and by oncogenic p21ras. We were therefore interested in determining the relationship between MAP kinases and raf. The MAP kinase ERK2 is activated by expression of oncogenically activated raf, independently of cellular ras. Overexpressed p74raf-1 potentiates activation of ERK2 by EGF and TPA. MAP kinase kinase inactivated by phosphatase 2A treatment is phosphorylated and reactivated by incubation with p74raf-1 immunoprecipitated from phorbol estertreated cells. We conclude that raf protein kinase is upstream of MAP kinases and is either a MAP kinase kinase kinase or a MAP kinase kinase kinase kinase.

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SN - 0092-8674

VL - 71

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JO - Cell

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Activation of the MAP kinase pathway by the protein kinase raf

Abstract

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