Activation of the neutrophil NADPH oxidase is inhibited by SB 203580, a specific inhibitor of SAPK2/p38

Aroon S. Lal, Andrew D. Clifton, John Rouse, Anthony W. Segal, Philip Cohen

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Activation of the neutrophil NADPH oxidase by either the bacterial peptide fMLP or phorbol myristate acetate (PMA) is partially suppressed by SB 203580, a specific inhibitor of the MAP kinase family member, SAPK2/p38. The concentration of SB 203580 that suppresses activation of NADPH oxidase is similar to that which inhibits SAPK2/p38 in vitro, and both fMLP and PMA induce an extremely rapid and potent activation of SAPK2/p38 in neutrophils. SB 203580 does not exert its effect by preventing the neutrophil priming reaction, by suppressing the phosphorylation of p47(phax), or by preventing the translocation of p47(phax)/67(phax) to the plasma membrane.

Original languageEnglish
Pages (from-to)465-470
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume259
Issue number2
DOIs
Publication statusPublished - 7 Jun 1999

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Mitogen-Activated Protein Kinase 11
Neutrophil Activation
NADPH Oxidase
Chemical activation
Tetradecanoylphorbol Acetate
Neutrophils
Phosphorylation
Cell membranes
Phosphotransferases
Cell Membrane
Peptides
SB 203580

Cite this

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title = "Activation of the neutrophil NADPH oxidase is inhibited by SB 203580, a specific inhibitor of SAPK2/p38",
abstract = "Activation of the neutrophil NADPH oxidase by either the bacterial peptide fMLP or phorbol myristate acetate (PMA) is partially suppressed by SB 203580, a specific inhibitor of the MAP kinase family member, SAPK2/p38. The concentration of SB 203580 that suppresses activation of NADPH oxidase is similar to that which inhibits SAPK2/p38 in vitro, and both fMLP and PMA induce an extremely rapid and potent activation of SAPK2/p38 in neutrophils. SB 203580 does not exert its effect by preventing the neutrophil priming reaction, by suppressing the phosphorylation of p47(phax), or by preventing the translocation of p47(phax)/67(phax) to the plasma membrane.",
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Activation of the neutrophil NADPH oxidase is inhibited by SB 203580, a specific inhibitor of SAPK2/p38. / Lal, Aroon S.; Clifton, Andrew D.; Rouse, John; Segal, Anthony W.; Cohen, Philip.

In: Biochemical and Biophysical Research Communications, Vol. 259, No. 2, 07.06.1999, p. 465-470.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Activation of the neutrophil NADPH oxidase is inhibited by SB 203580, a specific inhibitor of SAPK2/p38

AU - Lal, Aroon S.

AU - Clifton, Andrew D.

AU - Rouse, John

AU - Segal, Anthony W.

AU - Cohen, Philip

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