TY - JOUR
T1 - Activators of protein kinase C down-regulate and phosphorylate the T3/T-cell antigen receptor complex of human T lymphocytes
AU - Cantrell, D. A.
AU - Davies, A. A.
AU - Crumpton, M. J.
PY - 1985/12/1
Y1 - 1985/12/1
N2 - As judged by indirect immunofluorescence, phorbol 12,13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using 125I-labeled antibody indicated that the reductin in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 M(r) 26,000 γ chain and to a lesser extent of the M(r) 21,000 δ chain. No phosphorylation of the T3 ε chain or of the α and β subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 γ and δ chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.
AB - As judged by indirect immunofluorescence, phorbol 12,13-dibutyrate and 1-oleoyl-2-acetylglycerol induced a rapid, concentration-dependent decrease of about 50% in the surface expression of the T3 antigen on human T lymphoblasts, and of T3 and the T-cell antigen receptor on HPB-ALL cells. Direct binding experiments using 125I-labeled antibody indicated that the reductin in T3 expression corresponded to a decrease in the number of antigen molecules rather than a change in their affinity. Biochemical analyses revealed that phorbol dibutyrate induced a rapid, prominent phosphorylation of the T3 M(r) 26,000 γ chain and to a lesser extent of the M(r) 21,000 δ chain. No phosphorylation of the T3 ε chain or of the α and β subunits of the T-cell antigen receptor was detected. The data suggest that protein kinase C induces a phosphorylation of the T3 γ and δ chains that may lead to the down-regulation of the T3/T-cell antigen receptor complex.
UR - http://www.scopus.com/inward/record.url?scp=0000351142&partnerID=8YFLogxK
U2 - 10.1073/pnas.82.23.8158
DO - 10.1073/pnas.82.23.8158
M3 - Article
C2 - 3934668
AN - SCOPUS:0000351142
SN - 0027-8424
VL - 82
SP - 8158
EP - 8162
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 23
ER -