Active-site topologies of human CYP2D6 and its aspartate-301 → glutamate, asparagine, and glycine mutants

Richard Mackman; Richard A. Tschirret-Guth; Gillian Smith; Graham P. Hayhurst; S. Wynne Ellis; Martin S. Lennard; Geoffrey T. Tucker; C. Roland Wolf; Paul R. Ortiz de Montellano

doi:10.1006/abbi.1996.0291

Active-site topologies of human CYP2D6 and its aspartate-301 → glutamate, asparagine, and glycine mutants

Richard Mackman, Richard A. Tschirret-Guth, Gillian Smith, Graham P. Hayhurst, S. Wynne Ellis, Martin S. Lennard, Geoffrey T. Tucker, C. Roland Wolf, Paul R. Ortiz de Montellano

Research output: Contribution to journal › Article

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Abstract

Cytochrome P450 2D6 (CYP2D6) catalyzes the oxidation of substrates with a positively charged nitrogen atom 5-7 angstroms from the site of the oxidation. The active-site topology of CYP2D6 is examined here with phenyl-, 2-naphthyl-, and p-biphenyldiazene, which react with P450 enzymes to form sigma-bonded aryl-iron (Fe-Ar) complexes. Ferricyanide-mediated migration of the aryl group from the iron to the porphyrin nitrogens produces the N-arylprotoporphyrin IX regioisomers (NB:NA:NC:ND, in which the aryl group is bound to the nitrogen of pyrrole rings B, A, C, and D, respectively) in the following ratios (zero means

Original language	English
Pages (from-to)	134-140
Number of pages	7
Journal	Archives of Biochemistry and Biophysics
Volume	331
Issue number	1
DOIs	https://doi.org/10.1006/abbi.1996.0291
Publication status	Published - 1996

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Mackman, R., Tschirret-Guth, R. A., Smith, G., Hayhurst, G. P., Ellis, S. W., Lennard, M. S., Tucker, G. T., Wolf, C. R., & Ortiz de Montellano, P. R. (1996). Active-site topologies of human CYP2D6 and its aspartate-301 → glutamate, asparagine, and glycine mutants. Archives of Biochemistry and Biophysics, 331(1), 134-140. https://doi.org/10.1006/abbi.1996.0291

Mackman, Richard ; Tschirret-Guth, Richard A. ; Smith, Gillian ; Hayhurst, Graham P. ; Ellis, S. Wynne ; Lennard, Martin S. ; Tucker, Geoffrey T. ; Wolf, C. Roland ; Ortiz de Montellano, Paul R. / Active-site topologies of human CYP2D6 and its aspartate-301 → glutamate, asparagine, and glycine mutants. In: Archives of Biochemistry and Biophysics. 1996 ; Vol. 331, No. 1. pp. 134-140.

@article{ee603faf08114c9b96f44416964cab29,

title = "Active-site topologies of human CYP2D6 and its aspartate-301 → glutamate, asparagine, and glycine mutants",

abstract = "Cytochrome P450 2D6 (CYP2D6) catalyzes the oxidation of substrates with a positively charged nitrogen atom 5-7 angstroms from the site of the oxidation. The active-site topology of CYP2D6 is examined here with phenyl-, 2-naphthyl-, and p-biphenyldiazene, which react with P450 enzymes to form sigma-bonded aryl-iron (Fe-Ar) complexes. Ferricyanide-mediated migration of the aryl group from the iron to the porphyrin nitrogens produces the N-arylprotoporphyrin IX regioisomers (NB:NA:NC:ND, in which the aryl group is bound to the nitrogen of pyrrole rings B, A, C, and D, respectively) in the following ratios (zero means ",

author = "Richard Mackman and Tschirret-Guth, {Richard A.} and Gillian Smith and Hayhurst, {Graham P.} and Ellis, {S. Wynne} and Lennard, {Martin S.} and Tucker, {Geoffrey T.} and Wolf, {C. Roland} and {Ortiz de Montellano}, {Paul R.}",

note = "This work was supported by National Institutes of Health Grant GM25515 (P.R.O.M.) and the Wellcome Trust Grant 038735 (M.S.L., G.T.T., C.R.W.). Support for the Liver Center core facilities at the University of California, San Francisco, was provided by Grant 5 P30 DK26743",

year = "1996",

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volume = "331",

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Active-site topologies of human CYP2D6 and its aspartate-301 → glutamate, asparagine, and glycine mutants. / Mackman, Richard; Tschirret-Guth, Richard A.; Smith, Gillian; Hayhurst, Graham P.; Ellis, S. Wynne; Lennard, Martin S.; Tucker, Geoffrey T.; Wolf, C. Roland; Ortiz de Montellano, Paul R.

In: Archives of Biochemistry and Biophysics, Vol. 331, No. 1, 1996, p. 134-140.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Active-site topologies of human CYP2D6 and its aspartate-301 → glutamate, asparagine, and glycine mutants

AU - Mackman, Richard

AU - Tschirret-Guth, Richard A.

AU - Smith, Gillian

AU - Hayhurst, Graham P.

AU - Ellis, S. Wynne

AU - Lennard, Martin S.

AU - Tucker, Geoffrey T.

AU - Wolf, C. Roland

AU - Ortiz de Montellano, Paul R.

N1 - This work was supported by National Institutes of Health Grant GM25515 (P.R.O.M.) and the Wellcome Trust Grant 038735 (M.S.L., G.T.T., C.R.W.). Support for the Liver Center core facilities at the University of California, San Francisco, was provided by Grant 5 P30 DK26743

PY - 1996

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U2 - 10.1006/abbi.1996.0291

DO - 10.1006/abbi.1996.0291

M3 - Article

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10.1006/abbi.1996.0291