Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1

Keith Baar, Adam R. Wende, Terry E. Jones, Matthew Marison, Lorraine A. Nolte, May Chen, Daniel P. Kelly, John O. Holloszy

    Research output: Contribution to journalArticle

    660 Citations (Scopus)

    Abstract

    Endurance exercise induces increases in mitochondria and the GLUT4 isoform of the glucose transporter in muscle. Although little is known about the mechanisms underlying these adaptations, new information has accumulated regarding how mitochondrial biogenesis and GLUT4 expression are regulated. This includes the findings that the transcriptional coactivator PGC-1 promotes mitochondrial biogenesis and that NRF-1 and NRF-2 act as transcriptional activators of genes encoding mitochondrial enzymes. We tested the hypothesis that increases in PGC-1, NRF-1, and NRF-2 are involved in the initial adaptive response of muscle to exercise. Five daily bouts of swimming induced increases in mitochondrial enzymes and GLUT4 in skeletal muscle in rats. One exercise bout resulted in ~ twofold increases in full-length muscle PGC-1 mRNA and PGC-1 protein, which were evident 18 h after exercise. A smaller form of PGC-1 increased after exercise. The exercise induced increases in muscle NRF-1 and NRF-2 that were evident 12 to 18 h after one exercise bout. These findings suggest that increases in PGC-1, NRF-1, and NRF-2 represent key regulatory components of the stimulation of mitochondrial biogenesis by exercise and that PGC-1 mediates the coordinated increases in GLUT4 and mitochondria.—Baar, K., Wende, A. R., Jones, T. E., Marison, M., Nolte, L. A., Chen, M., Kelly, D. P., Holloszy, J. O. Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1.
    Original languageEnglish
    Pages (from-to)1879-1886
    Number of pages8
    JournalFASEB Journal
    Volume16
    Issue number14
    DOIs
    Publication statusPublished - Dec 2002

    Fingerprint

    Muscle
    Skeletal Muscle
    Organelle Biogenesis
    Muscles
    Mitochondria
    Mitochondrial Genes
    Facilitative Glucose Transport Proteins
    Enzymes
    Protein Isoforms
    Gene encoding
    Messenger RNA
    Rats
    Durability
    Proteins

    Keywords

    • Skeletal muscle
    • Exercise
    • Mitochondria

    Cite this

    Baar, K., Wende, A. R., Jones, T. E., Marison, M., Nolte, L. A., Chen, M., ... Holloszy, J. O. (2002). Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1. FASEB Journal, 16(14), 1879-1886. https://doi.org/10.1096/fj.02-0367com
    Baar, Keith ; Wende, Adam R. ; Jones, Terry E. ; Marison, Matthew ; Nolte, Lorraine A. ; Chen, May ; Kelly, Daniel P. ; Holloszy, John O. / Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1. In: FASEB Journal. 2002 ; Vol. 16, No. 14. pp. 1879-1886.
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    Baar, K, Wende, AR, Jones, TE, Marison, M, Nolte, LA, Chen, M, Kelly, DP & Holloszy, JO 2002, 'Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1', FASEB Journal, vol. 16, no. 14, pp. 1879-1886. https://doi.org/10.1096/fj.02-0367com

    Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1. / Baar, Keith; Wende, Adam R.; Jones, Terry E.; Marison, Matthew; Nolte, Lorraine A.; Chen, May; Kelly, Daniel P.; Holloszy, John O.

    In: FASEB Journal, Vol. 16, No. 14, 12.2002, p. 1879-1886.

    Research output: Contribution to journalArticle

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    T1 - Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1

    AU - Baar, Keith

    AU - Wende, Adam R.

    AU - Jones, Terry E.

    AU - Marison, Matthew

    AU - Nolte, Lorraine A.

    AU - Chen, May

    AU - Kelly, Daniel P.

    AU - Holloszy, John O.

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    PY - 2002/12

    Y1 - 2002/12

    N2 - Endurance exercise induces increases in mitochondria and the GLUT4 isoform of the glucose transporter in muscle. Although little is known about the mechanisms underlying these adaptations, new information has accumulated regarding how mitochondrial biogenesis and GLUT4 expression are regulated. This includes the findings that the transcriptional coactivator PGC-1 promotes mitochondrial biogenesis and that NRF-1 and NRF-2 act as transcriptional activators of genes encoding mitochondrial enzymes. We tested the hypothesis that increases in PGC-1, NRF-1, and NRF-2 are involved in the initial adaptive response of muscle to exercise. Five daily bouts of swimming induced increases in mitochondrial enzymes and GLUT4 in skeletal muscle in rats. One exercise bout resulted in ~ twofold increases in full-length muscle PGC-1 mRNA and PGC-1 protein, which were evident 18 h after exercise. A smaller form of PGC-1 increased after exercise. The exercise induced increases in muscle NRF-1 and NRF-2 that were evident 12 to 18 h after one exercise bout. These findings suggest that increases in PGC-1, NRF-1, and NRF-2 represent key regulatory components of the stimulation of mitochondrial biogenesis by exercise and that PGC-1 mediates the coordinated increases in GLUT4 and mitochondria.—Baar, K., Wende, A. R., Jones, T. E., Marison, M., Nolte, L. A., Chen, M., Kelly, D. P., Holloszy, J. O. Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1.

    AB - Endurance exercise induces increases in mitochondria and the GLUT4 isoform of the glucose transporter in muscle. Although little is known about the mechanisms underlying these adaptations, new information has accumulated regarding how mitochondrial biogenesis and GLUT4 expression are regulated. This includes the findings that the transcriptional coactivator PGC-1 promotes mitochondrial biogenesis and that NRF-1 and NRF-2 act as transcriptional activators of genes encoding mitochondrial enzymes. We tested the hypothesis that increases in PGC-1, NRF-1, and NRF-2 are involved in the initial adaptive response of muscle to exercise. Five daily bouts of swimming induced increases in mitochondrial enzymes and GLUT4 in skeletal muscle in rats. One exercise bout resulted in ~ twofold increases in full-length muscle PGC-1 mRNA and PGC-1 protein, which were evident 18 h after exercise. A smaller form of PGC-1 increased after exercise. The exercise induced increases in muscle NRF-1 and NRF-2 that were evident 12 to 18 h after one exercise bout. These findings suggest that increases in PGC-1, NRF-1, and NRF-2 represent key regulatory components of the stimulation of mitochondrial biogenesis by exercise and that PGC-1 mediates the coordinated increases in GLUT4 and mitochondria.—Baar, K., Wende, A. R., Jones, T. E., Marison, M., Nolte, L. A., Chen, M., Kelly, D. P., Holloszy, J. O. Adaptations of skeletal muscle to exercise: rapid increase in the transcriptional coactivator PGC-1.

    KW - Skeletal muscle

    KW - Exercise

    KW - Mitochondria

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    DO - 10.1096/fj.02-0367com

    M3 - Article

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    SN - 0892-6638

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