ADS1 encodes a MATE-transporter that negatively regulates plant disease resistance

Xinli Sun, Eleanor M. Gilroy, Andrea Chini, Pedro L. Nurmberg, Ingo Hein, Christophe Lacomme, Paul R. J. Birch, Adil Hussain, Byung-Wook Yun, Gary J. Loake

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    29 Citations (Scopus)

    Abstract

    Multidrug and toxic compound extrusion (MATE) proteins comprise the most recently identified family of multidrug transporters. In plants, the numbers of MATE proteins has undergone a remarkable expansion, underscoring the importance of these transporters within this kingdom.

    Here, we describe the identification and characterization of Activated Disease Susceptibility 1 (ADS1) which encodes a putative MATE transport protein. An activation tagging screen uncovered the ads1-Dominant (ads1-D) mutant, which was subsequently characterized by molecular, genetic and biochemical approaches.

    The ads1-D mutant was compromised in both basal and nonhost resistance against microbial pathogens. Further, plant defence responses conferred by RPS4 were also disabled in ads1-D plants. By contrast, depletion of ADS1 transcripts by RNA-interference (RNAi) promoted basal disease resistance. Unexpectedly, ads1-D plants were found to constitutively accumulate reactive oxygen intermediates (ROIs). However, analysis of ads1-D Arabidopsis thaliana respiratory burst oxidase (atrboh) double and triple mutants indicated that an increase in ROIs did not impact ads1-D-mediated disease susceptibility.

    Our findings imply that ADS1 negatively regulates the accumulation of the plant immune activator salicylic acid (SA) and cognate Pathogenesis-Related 1 (PR1) gene expression. Collectively, these data highlight an important role for MATE proteins in the establishment of plant disease resistance.

    Original languageEnglish
    Pages (from-to)471-482
    Number of pages12
    JournalNew Phytologist
    Volume192
    Issue number2
    DOIs
    Publication statusPublished - 2011

    Keywords

    • activation tagging
    • biotic stress and defence signalling
    • disease resistance
    • MATE protein
    • plant defence
    • SYSTEMIC ACQUIRED-RESISTANCE
    • SALICYLIC-ACID INDUCTION
    • NBS-LRR GENE
    • NONHOST RESISTANCE
    • TRANSCRIPTION FACTORS
    • PSEUDOMONAS-SYRINGAE
    • ARABIDOPSIS-THALIANA
    • OXIDATIVE BURST
    • ORGANIC CATIONS
    • PROTEIN

    Cite this

    Sun, X., Gilroy, E. M., Chini, A., Nurmberg, P. L., Hein, I., Lacomme, C., Birch, P. R. J., Hussain, A., Yun, B-W., & Loake, G. J. (2011). ADS1 encodes a MATE-transporter that negatively regulates plant disease resistance. New Phytologist, 192(2), 471-482. https://doi.org/10.1111/j.1469-8137.2011.03820.x