Agonist potency determination by patch clamp analysis of single glutamate receptors

Kenneth A. F. Gration; Jeremy J. Lambert; Robert L. Ramsey; R. Peter Rand; Peter N. R. Usherwood

doi:10.1016/0006-8993(81)90423-6

Agonist potency determination by patch clamp analysis of single glutamate receptors

Kenneth A. F. Gration, Jeremy J. Lambert, Robert L. Ramsey, R. Peter Rand, Peter N. R. Usherwood

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

Agonists (l-quisqualate, l-glutamate and l-cysteine sulphinate) of the locust muscle glutamate receptor differ in potency. Patch clamp analysis of single glutamate receptors reveals that the relative potencies of agonists are determined both by the life-times of the channels that they gate and their probability of activating the glutamate receptor-ionophore complex. Furthermore, agonists which most readily activate the receptor channel complex gate channels with longer life-times than agonists which are less efficient in this respect.

Original language	English
Pages (from-to)	400-405
Number of pages	6
Journal	Brain Research
Volume	230
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(81)90423-6
Publication status	Published - 28 Dec 1981

Keywords

agonist potency
glutamate agonist
glutamate channel
l-cysteine sulphinate
l-glutamate
l-quisqualate
patch clamp analysis

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Developmental Biology
Clinical Neurology

Access to Document

10.1016/0006-8993(81)90423-6

Cite this

@article{e36c406d1c014530a0a73e62efea3d96,

title = "Agonist potency determination by patch clamp analysis of single glutamate receptors",

abstract = "Agonists (l-quisqualate, l-glutamate and l-cysteine sulphinate) of the locust muscle glutamate receptor differ in potency. Patch clamp analysis of single glutamate receptors reveals that the relative potencies of agonists are determined both by the life-times of the channels that they gate and their probability of activating the glutamate receptor-ionophore complex. Furthermore, agonists which most readily activate the receptor channel complex gate channels with longer life-times than agonists which are less efficient in this respect.",

keywords = "agonist potency, glutamate agonist, glutamate channel, l-cysteine sulphinate, l-glutamate, l-quisqualate, patch clamp analysis",

author = "Gration, {Kenneth A. F.} and Lambert, {Jeremy J.} and Ramsey, {Robert L.} and Rand, {R. Peter} and Usherwood, {Peter N. R.}",

year = "1981",

month = dec,

day = "28",

doi = "10.1016/0006-8993(81)90423-6",

language = "English",

volume = "230",

pages = "400--405",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Agonist potency determination by patch clamp analysis of single glutamate receptors

AU - Gration, Kenneth A. F.

AU - Lambert, Jeremy J.

AU - Ramsey, Robert L.

AU - Rand, R. Peter

AU - Usherwood, Peter N. R.

PY - 1981/12/28

Y1 - 1981/12/28

N2 - Agonists (l-quisqualate, l-glutamate and l-cysteine sulphinate) of the locust muscle glutamate receptor differ in potency. Patch clamp analysis of single glutamate receptors reveals that the relative potencies of agonists are determined both by the life-times of the channels that they gate and their probability of activating the glutamate receptor-ionophore complex. Furthermore, agonists which most readily activate the receptor channel complex gate channels with longer life-times than agonists which are less efficient in this respect.

AB - Agonists (l-quisqualate, l-glutamate and l-cysteine sulphinate) of the locust muscle glutamate receptor differ in potency. Patch clamp analysis of single glutamate receptors reveals that the relative potencies of agonists are determined both by the life-times of the channels that they gate and their probability of activating the glutamate receptor-ionophore complex. Furthermore, agonists which most readily activate the receptor channel complex gate channels with longer life-times than agonists which are less efficient in this respect.

KW - agonist potency

KW - glutamate agonist

KW - glutamate channel

KW - l-cysteine sulphinate

KW - l-glutamate

KW - l-quisqualate

KW - patch clamp analysis

U2 - 10.1016/0006-8993(81)90423-6

DO - 10.1016/0006-8993(81)90423-6

M3 - Article

C2 - 6119138

AN - SCOPUS:0019817543

SN - 0006-8993

VL - 230

SP - 400

EP - 405

JO - Brain Research

JF - Brain Research

IS - 1-2

ER -

Agonist potency determination by patch clamp analysis of single glutamate receptors

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Fingerprint

Cite this