ALADIN is Required for the Production of Fertile Mouse Oocytes

Sara Carvalhal, Michelle Stevense, Katrin Koehler, Ronald Naumann, Angela Huebner, Rolf Jessberger, Eric R. Griffis (Lead / Corresponding author)

Research output: Contribution to journalArticle

6 Citations (Scopus)
101 Downloads (Pure)

Abstract

Asymmetric cell divisions depend upon the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small, non-developing polar bodies at anaphase. By dividing so asymmetrically, most of the cytoplasmic content within the oocyte is preserved, which is critical for successful fertilization and early development. Recently, we determined that the nucleoporin ALADIN participates in spindle assembly in somatic cells, and we have also shown that female mice homozygously null for ALADIN are sterile. In this study we show that this protein is involved in specific meiotic stages including meiotic resumption, spindle assembly, and spindle positioning. In the absence of ALADIN, polar body extrusion is compromised due to problems in spindle orientation and anchoring at the first meiotic anaphase. ALADIN null oocytes that mature far enough to be fertilized in vitro are unable to support embryonic development beyond the two-cell stage. Overall, we find that ALADIN is critical for oocyte maturation and appears to be far more essential for this process than for somatic cell divisions.

Original languageEnglish
Pages (from-to)2470-2478
Number of pages9
JournalMolecular Biology of the Cell
Volume28
Issue number19
Early online date2 Aug 2017
DOIs
Publication statusPublished - 17 Sep 2017

Fingerprint

Oocytes
Polar Bodies
Anaphase
Spindle Apparatus
Asymmetric Cell Division
Nuclear Pore Complex Proteins
Chromosome Segregation
Fertilization
Cell Division
Embryonic Development
Chromosomes
Proteins

Keywords

  • ALADIN
  • Meiosis
  • Mouse oocyte maturation and asymmetric cell division

Cite this

Carvalhal, S., Stevense, M., Koehler, K., Naumann, R., Huebner, A., Jessberger, R., & Griffis, E. R. (2017). ALADIN is Required for the Production of Fertile Mouse Oocytes. Molecular Biology of the Cell, 28(19), 2470-2478. https://doi.org/10.1091/mbc.E16-03-0158
Carvalhal, Sara ; Stevense, Michelle ; Koehler, Katrin ; Naumann, Ronald ; Huebner, Angela ; Jessberger, Rolf ; Griffis, Eric R. / ALADIN is Required for the Production of Fertile Mouse Oocytes. In: Molecular Biology of the Cell. 2017 ; Vol. 28, No. 19. pp. 2470-2478.
@article{6dc513017998435eb009a23775d7111c,
title = "ALADIN is Required for the Production of Fertile Mouse Oocytes",
abstract = "Asymmetric cell divisions depend upon the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small, non-developing polar bodies at anaphase. By dividing so asymmetrically, most of the cytoplasmic content within the oocyte is preserved, which is critical for successful fertilization and early development. Recently, we determined that the nucleoporin ALADIN participates in spindle assembly in somatic cells, and we have also shown that female mice homozygously null for ALADIN are sterile. In this study we show that this protein is involved in specific meiotic stages including meiotic resumption, spindle assembly, and spindle positioning. In the absence of ALADIN, polar body extrusion is compromised due to problems in spindle orientation and anchoring at the first meiotic anaphase. ALADIN null oocytes that mature far enough to be fertilized in vitro are unable to support embryonic development beyond the two-cell stage. Overall, we find that ALADIN is critical for oocyte maturation and appears to be far more essential for this process than for somatic cell divisions.",
keywords = "ALADIN, Meiosis, Mouse oocyte maturation and asymmetric cell division",
author = "Sara Carvalhal and Michelle Stevense and Katrin Koehler and Ronald Naumann and Angela Huebner and Rolf Jessberger and Griffis, {Eric R.}",
note = "MS is supported through institutional funds and DFG grant STE 2280/2-1, and AH and KK are supported by DFG grants HU 895/5-1 and HU 895/5-2. SC was supported by an EMBO Short Term Fellowship (ASTF 521-2014) and a Biotechnology and Biological Sciences Research Council studentship. RJ is supported by institutional funds and by DFG grant JE150/11-2. ERG was supported by a Wellcome Trust RCDF award (090064/Z/09/Z) and a Wellcome Trust Strategic award to the Centre for Gene Regulation and Expression (097945/B/11/Z).",
year = "2017",
month = "9",
day = "17",
doi = "10.1091/mbc.E16-03-0158",
language = "English",
volume = "28",
pages = "2470--2478",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "19",

}

Carvalhal, S, Stevense, M, Koehler, K, Naumann, R, Huebner, A, Jessberger, R & Griffis, ER 2017, 'ALADIN is Required for the Production of Fertile Mouse Oocytes', Molecular Biology of the Cell, vol. 28, no. 19, pp. 2470-2478. https://doi.org/10.1091/mbc.E16-03-0158

ALADIN is Required for the Production of Fertile Mouse Oocytes. / Carvalhal, Sara; Stevense, Michelle; Koehler, Katrin; Naumann, Ronald; Huebner, Angela; Jessberger, Rolf; Griffis, Eric R. (Lead / Corresponding author).

In: Molecular Biology of the Cell, Vol. 28, No. 19, 17.09.2017, p. 2470-2478.

Research output: Contribution to journalArticle

TY - JOUR

T1 - ALADIN is Required for the Production of Fertile Mouse Oocytes

AU - Carvalhal, Sara

AU - Stevense, Michelle

AU - Koehler, Katrin

AU - Naumann, Ronald

AU - Huebner, Angela

AU - Jessberger, Rolf

AU - Griffis, Eric R.

N1 - MS is supported through institutional funds and DFG grant STE 2280/2-1, and AH and KK are supported by DFG grants HU 895/5-1 and HU 895/5-2. SC was supported by an EMBO Short Term Fellowship (ASTF 521-2014) and a Biotechnology and Biological Sciences Research Council studentship. RJ is supported by institutional funds and by DFG grant JE150/11-2. ERG was supported by a Wellcome Trust RCDF award (090064/Z/09/Z) and a Wellcome Trust Strategic award to the Centre for Gene Regulation and Expression (097945/B/11/Z).

PY - 2017/9/17

Y1 - 2017/9/17

N2 - Asymmetric cell divisions depend upon the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small, non-developing polar bodies at anaphase. By dividing so asymmetrically, most of the cytoplasmic content within the oocyte is preserved, which is critical for successful fertilization and early development. Recently, we determined that the nucleoporin ALADIN participates in spindle assembly in somatic cells, and we have also shown that female mice homozygously null for ALADIN are sterile. In this study we show that this protein is involved in specific meiotic stages including meiotic resumption, spindle assembly, and spindle positioning. In the absence of ALADIN, polar body extrusion is compromised due to problems in spindle orientation and anchoring at the first meiotic anaphase. ALADIN null oocytes that mature far enough to be fertilized in vitro are unable to support embryonic development beyond the two-cell stage. Overall, we find that ALADIN is critical for oocyte maturation and appears to be far more essential for this process than for somatic cell divisions.

AB - Asymmetric cell divisions depend upon the precise placement of the spindle apparatus. In mammalian oocytes, spindles assemble close to the cell's center but chromosome segregation takes place at the cell periphery where half of the chromosomes are expelled into small, non-developing polar bodies at anaphase. By dividing so asymmetrically, most of the cytoplasmic content within the oocyte is preserved, which is critical for successful fertilization and early development. Recently, we determined that the nucleoporin ALADIN participates in spindle assembly in somatic cells, and we have also shown that female mice homozygously null for ALADIN are sterile. In this study we show that this protein is involved in specific meiotic stages including meiotic resumption, spindle assembly, and spindle positioning. In the absence of ALADIN, polar body extrusion is compromised due to problems in spindle orientation and anchoring at the first meiotic anaphase. ALADIN null oocytes that mature far enough to be fertilized in vitro are unable to support embryonic development beyond the two-cell stage. Overall, we find that ALADIN is critical for oocyte maturation and appears to be far more essential for this process than for somatic cell divisions.

KW - ALADIN

KW - Meiosis

KW - Mouse oocyte maturation and asymmetric cell division

U2 - 10.1091/mbc.E16-03-0158

DO - 10.1091/mbc.E16-03-0158

M3 - Article

C2 - 28768824

VL - 28

SP - 2470

EP - 2478

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 19

ER -

Carvalhal S, Stevense M, Koehler K, Naumann R, Huebner A, Jessberger R et al. ALADIN is Required for the Production of Fertile Mouse Oocytes. Molecular Biology of the Cell. 2017 Sep 17;28(19):2470-2478. https://doi.org/10.1091/mbc.E16-03-0158