Alfalfa heat shock genes are differentially expressed during somatic embryogenesis

János Györgyey, Anton Gartner, Kinga Németh, Zoltán Magyar, Heribert Hirt, Erwin Heberle-Bors, Dénes Dudits (Lead / Corresponding author)

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

We have isolated two cDNA clones (Mshsp18-1; Mshsp18-2) from alfalfa (Medicago sativa L.) which encode for small heat shock proteins (HSPs) belonging to the hsp17 subfamily. The predicted amino acid sequences of the two alfalfa proteins are 92% identical and a similar degree of homology (90%) can be detected between Mshsp 18-2 and the pea hsp 17. In comparison to various members of small HSPs from soybean amino acid sequence similarities of 80-86% were identified. The alfalfa HSPs share a homologous stretch of amino acids in the carboxy terminal region with hsp22, 23, 26 from Drosophila. This region contains the GVLTV motif which is characteristic of several members of small HSPs. At room temperature alfalfa hsp 18 mRNAs were not detectable in root and leaf tissues but northern analysis showed a low level of expression in microcallus suspension (MCS). The transcription of Mshsp 18 genes is induced by elevated temperature, CdCl2 treatment and osmotic shock in cultured cells. In alfalfa somatic embryos derived from MCS a considerable amount of hsp 18 mRNA can be detected during the early embryogenic stages under normal culture conditions. The differential expression of these genes during embryo development suggests a specific functional role for HSPs in plant cells at the time of the developmental switch in vitro.

Original languageEnglish
Pages (from-to)999-1007
Number of pages9
JournalPlant Molecular Biology
Volume16
Issue number6
DOIs
Publication statusPublished - 1 Jun 1991

Fingerprint

Medicago sativa
somatic embryogenesis
heat shock proteins
Embryonic Development
heat stress
alfalfa
Shock
Hot Temperature
Small Heat-Shock Proteins
alfalfa protein
Genes
genes
Heat-Shock Proteins
amino acid sequences
Amino Acid Sequence
Suspensions
Cadmium Chloride
Messenger RNA
osmotic stress
Temperature

Keywords

  • heat shock protein (HSP)
  • Medicago sativa L.
  • somatic embryogenesis
  • stress response
  • tissue culture

Cite this

Györgyey, J., Gartner, A., Németh, K., Magyar, Z., Hirt, H., Heberle-Bors, E., & Dudits, D. (1991). Alfalfa heat shock genes are differentially expressed during somatic embryogenesis. Plant Molecular Biology, 16(6), 999-1007. https://doi.org/10.1007/BF00016072
Györgyey, János ; Gartner, Anton ; Németh, Kinga ; Magyar, Zoltán ; Hirt, Heribert ; Heberle-Bors, Erwin ; Dudits, Dénes. / Alfalfa heat shock genes are differentially expressed during somatic embryogenesis. In: Plant Molecular Biology. 1991 ; Vol. 16, No. 6. pp. 999-1007.
@article{2258a98d6559413784a6771f118acf01,
title = "Alfalfa heat shock genes are differentially expressed during somatic embryogenesis",
abstract = "We have isolated two cDNA clones (Mshsp18-1; Mshsp18-2) from alfalfa (Medicago sativa L.) which encode for small heat shock proteins (HSPs) belonging to the hsp17 subfamily. The predicted amino acid sequences of the two alfalfa proteins are 92{\%} identical and a similar degree of homology (90{\%}) can be detected between Mshsp 18-2 and the pea hsp 17. In comparison to various members of small HSPs from soybean amino acid sequence similarities of 80-86{\%} were identified. The alfalfa HSPs share a homologous stretch of amino acids in the carboxy terminal region with hsp22, 23, 26 from Drosophila. This region contains the GVLTV motif which is characteristic of several members of small HSPs. At room temperature alfalfa hsp 18 mRNAs were not detectable in root and leaf tissues but northern analysis showed a low level of expression in microcallus suspension (MCS). The transcription of Mshsp 18 genes is induced by elevated temperature, CdCl2 treatment and osmotic shock in cultured cells. In alfalfa somatic embryos derived from MCS a considerable amount of hsp 18 mRNA can be detected during the early embryogenic stages under normal culture conditions. The differential expression of these genes during embryo development suggests a specific functional role for HSPs in plant cells at the time of the developmental switch in vitro.",
keywords = "heat shock protein (HSP), Medicago sativa L., somatic embryogenesis, stress response, tissue culture",
author = "J{\'a}nos Gy{\"o}rgyey and Anton Gartner and Kinga N{\'e}meth and Zolt{\'a}n Magyar and Heribert Hirt and Erwin Heberle-Bors and D{\'e}nes Dudits",
year = "1991",
month = "6",
day = "1",
doi = "10.1007/BF00016072",
language = "English",
volume = "16",
pages = "999--1007",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer Verlag",
number = "6",

}

Györgyey, J, Gartner, A, Németh, K, Magyar, Z, Hirt, H, Heberle-Bors, E & Dudits, D 1991, 'Alfalfa heat shock genes are differentially expressed during somatic embryogenesis', Plant Molecular Biology, vol. 16, no. 6, pp. 999-1007. https://doi.org/10.1007/BF00016072

Alfalfa heat shock genes are differentially expressed during somatic embryogenesis. / Györgyey, János; Gartner, Anton; Németh, Kinga; Magyar, Zoltán; Hirt, Heribert; Heberle-Bors, Erwin; Dudits, Dénes (Lead / Corresponding author).

In: Plant Molecular Biology, Vol. 16, No. 6, 01.06.1991, p. 999-1007.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Alfalfa heat shock genes are differentially expressed during somatic embryogenesis

AU - Györgyey, János

AU - Gartner, Anton

AU - Németh, Kinga

AU - Magyar, Zoltán

AU - Hirt, Heribert

AU - Heberle-Bors, Erwin

AU - Dudits, Dénes

PY - 1991/6/1

Y1 - 1991/6/1

N2 - We have isolated two cDNA clones (Mshsp18-1; Mshsp18-2) from alfalfa (Medicago sativa L.) which encode for small heat shock proteins (HSPs) belonging to the hsp17 subfamily. The predicted amino acid sequences of the two alfalfa proteins are 92% identical and a similar degree of homology (90%) can be detected between Mshsp 18-2 and the pea hsp 17. In comparison to various members of small HSPs from soybean amino acid sequence similarities of 80-86% were identified. The alfalfa HSPs share a homologous stretch of amino acids in the carboxy terminal region with hsp22, 23, 26 from Drosophila. This region contains the GVLTV motif which is characteristic of several members of small HSPs. At room temperature alfalfa hsp 18 mRNAs were not detectable in root and leaf tissues but northern analysis showed a low level of expression in microcallus suspension (MCS). The transcription of Mshsp 18 genes is induced by elevated temperature, CdCl2 treatment and osmotic shock in cultured cells. In alfalfa somatic embryos derived from MCS a considerable amount of hsp 18 mRNA can be detected during the early embryogenic stages under normal culture conditions. The differential expression of these genes during embryo development suggests a specific functional role for HSPs in plant cells at the time of the developmental switch in vitro.

AB - We have isolated two cDNA clones (Mshsp18-1; Mshsp18-2) from alfalfa (Medicago sativa L.) which encode for small heat shock proteins (HSPs) belonging to the hsp17 subfamily. The predicted amino acid sequences of the two alfalfa proteins are 92% identical and a similar degree of homology (90%) can be detected between Mshsp 18-2 and the pea hsp 17. In comparison to various members of small HSPs from soybean amino acid sequence similarities of 80-86% were identified. The alfalfa HSPs share a homologous stretch of amino acids in the carboxy terminal region with hsp22, 23, 26 from Drosophila. This region contains the GVLTV motif which is characteristic of several members of small HSPs. At room temperature alfalfa hsp 18 mRNAs were not detectable in root and leaf tissues but northern analysis showed a low level of expression in microcallus suspension (MCS). The transcription of Mshsp 18 genes is induced by elevated temperature, CdCl2 treatment and osmotic shock in cultured cells. In alfalfa somatic embryos derived from MCS a considerable amount of hsp 18 mRNA can be detected during the early embryogenic stages under normal culture conditions. The differential expression of these genes during embryo development suggests a specific functional role for HSPs in plant cells at the time of the developmental switch in vitro.

KW - heat shock protein (HSP)

KW - Medicago sativa L.

KW - somatic embryogenesis

KW - stress response

KW - tissue culture

UR - http://www.scopus.com/inward/record.url?scp=0026181083&partnerID=8YFLogxK

U2 - 10.1007/BF00016072

DO - 10.1007/BF00016072

M3 - Article

C2 - 1863771

AN - SCOPUS:0026181083

VL - 16

SP - 999

EP - 1007

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 6

ER -