Allelism at the glutathione S-transferase GSTM3 locus

interactions with GSTM1 and GSTT1 as risk factors for astrocytoma

P A Hand, A Inskip, J Gilford, J Alldersea, J Elexpuru-Camiruaga, J D Hayes, P W Jones, R C Strange, A A Fryer

Research output: Contribution to journalArticle

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Abstract

We describe studies to assess the influence of polymorphism in the human glutathione S-transferase GSTM3 gene on susceptibility to high grade astrocytoma. Immunohistochemical studies using a GSTM3-specific antiserum identified expression of the GSTM3 subunit in astrocytes. The relative levels of expression of GSTM1 and GSTM3 in brain cytosols were determined after resolution of these enzymes using chromatofocusing. We found no differences in the level of GSTM3 activity in individuals with GSTM1 null and those with GSTM1-positive genotypes (GSTM1 A, GSTM1 B and GSTM1 A/B). A case-control study was performed to determine if GSTM3 alone or in combination with GSTM1 or GSTT1 influenced susceptibility to high grade astrocytoma. After correction for differences in age and gender, GSTM3 AA was not significantly different in cases compared with controls. No significant interactions between GSTM3 AA and GSTM1 null were identified. The significant interaction between GSTM3 AA and GSTT1 null appeared to result from the strength of the main effect (GSTT1 null). The data show that while GSTM3 is expressed in astrocytes and contributes significantly to total GST activity in human brain, it does not appear to influence susceptibility to high grade astrocytoma. Further, unlike lung, there appears to be no relationship between the level of GSTM3 activity in brain and GSTM1 genotype.

Original languageEnglish
Pages (from-to)1919-22
Number of pages4
JournalCarcinogenesis
Volume17
Issue number9
Publication statusPublished - Sep 1996

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Astrocytoma
Glutathione Transferase
Astrocytes
Brain
Genotype
Human Activities
Cytosol
Case-Control Studies
Immune Sera
Lung
Enzymes
Genes

Keywords

  • Alleles
  • Astrocytoma/epidemiology
  • Base Sequence
  • Brain/enzymology
  • Brain Neoplasms/epidemiology
  • Cytosol/enzymology
  • Gene Frequency
  • Genotype
  • Glutathione Transferase/genetics
  • Humans
  • Isoenzymes/genetics
  • Risk Factors

Cite this

Hand, P. A., Inskip, A., Gilford, J., Alldersea, J., Elexpuru-Camiruaga, J., Hayes, J. D., ... Fryer, A. A. (1996). Allelism at the glutathione S-transferase GSTM3 locus: interactions with GSTM1 and GSTT1 as risk factors for astrocytoma. Carcinogenesis, 17(9), 1919-22.
Hand, P A ; Inskip, A ; Gilford, J ; Alldersea, J ; Elexpuru-Camiruaga, J ; Hayes, J D ; Jones, P W ; Strange, R C ; Fryer, A A. / Allelism at the glutathione S-transferase GSTM3 locus : interactions with GSTM1 and GSTT1 as risk factors for astrocytoma. In: Carcinogenesis. 1996 ; Vol. 17, No. 9. pp. 1919-22.
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abstract = "We describe studies to assess the influence of polymorphism in the human glutathione S-transferase GSTM3 gene on susceptibility to high grade astrocytoma. Immunohistochemical studies using a GSTM3-specific antiserum identified expression of the GSTM3 subunit in astrocytes. The relative levels of expression of GSTM1 and GSTM3 in brain cytosols were determined after resolution of these enzymes using chromatofocusing. We found no differences in the level of GSTM3 activity in individuals with GSTM1 null and those with GSTM1-positive genotypes (GSTM1 A, GSTM1 B and GSTM1 A/B). A case-control study was performed to determine if GSTM3 alone or in combination with GSTM1 or GSTT1 influenced susceptibility to high grade astrocytoma. After correction for differences in age and gender, GSTM3 AA was not significantly different in cases compared with controls. No significant interactions between GSTM3 AA and GSTM1 null were identified. The significant interaction between GSTM3 AA and GSTT1 null appeared to result from the strength of the main effect (GSTT1 null). The data show that while GSTM3 is expressed in astrocytes and contributes significantly to total GST activity in human brain, it does not appear to influence susceptibility to high grade astrocytoma. Further, unlike lung, there appears to be no relationship between the level of GSTM3 activity in brain and GSTM1 genotype.",
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Hand, PA, Inskip, A, Gilford, J, Alldersea, J, Elexpuru-Camiruaga, J, Hayes, JD, Jones, PW, Strange, RC & Fryer, AA 1996, 'Allelism at the glutathione S-transferase GSTM3 locus: interactions with GSTM1 and GSTT1 as risk factors for astrocytoma', Carcinogenesis, vol. 17, no. 9, pp. 1919-22.

Allelism at the glutathione S-transferase GSTM3 locus : interactions with GSTM1 and GSTT1 as risk factors for astrocytoma. / Hand, P A; Inskip, A; Gilford, J; Alldersea, J; Elexpuru-Camiruaga, J; Hayes, J D; Jones, P W; Strange, R C; Fryer, A A.

In: Carcinogenesis, Vol. 17, No. 9, 09.1996, p. 1919-22.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Allelism at the glutathione S-transferase GSTM3 locus

T2 - interactions with GSTM1 and GSTT1 as risk factors for astrocytoma

AU - Hand, P A

AU - Inskip, A

AU - Gilford, J

AU - Alldersea, J

AU - Elexpuru-Camiruaga, J

AU - Hayes, J D

AU - Jones, P W

AU - Strange, R C

AU - Fryer, A A

PY - 1996/9

Y1 - 1996/9

N2 - We describe studies to assess the influence of polymorphism in the human glutathione S-transferase GSTM3 gene on susceptibility to high grade astrocytoma. Immunohistochemical studies using a GSTM3-specific antiserum identified expression of the GSTM3 subunit in astrocytes. The relative levels of expression of GSTM1 and GSTM3 in brain cytosols were determined after resolution of these enzymes using chromatofocusing. We found no differences in the level of GSTM3 activity in individuals with GSTM1 null and those with GSTM1-positive genotypes (GSTM1 A, GSTM1 B and GSTM1 A/B). A case-control study was performed to determine if GSTM3 alone or in combination with GSTM1 or GSTT1 influenced susceptibility to high grade astrocytoma. After correction for differences in age and gender, GSTM3 AA was not significantly different in cases compared with controls. No significant interactions between GSTM3 AA and GSTM1 null were identified. The significant interaction between GSTM3 AA and GSTT1 null appeared to result from the strength of the main effect (GSTT1 null). The data show that while GSTM3 is expressed in astrocytes and contributes significantly to total GST activity in human brain, it does not appear to influence susceptibility to high grade astrocytoma. Further, unlike lung, there appears to be no relationship between the level of GSTM3 activity in brain and GSTM1 genotype.

AB - We describe studies to assess the influence of polymorphism in the human glutathione S-transferase GSTM3 gene on susceptibility to high grade astrocytoma. Immunohistochemical studies using a GSTM3-specific antiserum identified expression of the GSTM3 subunit in astrocytes. The relative levels of expression of GSTM1 and GSTM3 in brain cytosols were determined after resolution of these enzymes using chromatofocusing. We found no differences in the level of GSTM3 activity in individuals with GSTM1 null and those with GSTM1-positive genotypes (GSTM1 A, GSTM1 B and GSTM1 A/B). A case-control study was performed to determine if GSTM3 alone or in combination with GSTM1 or GSTT1 influenced susceptibility to high grade astrocytoma. After correction for differences in age and gender, GSTM3 AA was not significantly different in cases compared with controls. No significant interactions between GSTM3 AA and GSTM1 null were identified. The significant interaction between GSTM3 AA and GSTT1 null appeared to result from the strength of the main effect (GSTT1 null). The data show that while GSTM3 is expressed in astrocytes and contributes significantly to total GST activity in human brain, it does not appear to influence susceptibility to high grade astrocytoma. Further, unlike lung, there appears to be no relationship between the level of GSTM3 activity in brain and GSTM1 genotype.

KW - Alleles

KW - Astrocytoma/epidemiology

KW - Base Sequence

KW - Brain/enzymology

KW - Brain Neoplasms/epidemiology

KW - Cytosol/enzymology

KW - Gene Frequency

KW - Genotype

KW - Glutathione Transferase/genetics

KW - Humans

KW - Isoenzymes/genetics

KW - Risk Factors

UR - https://www.ncbi.nlm.nih.gov/pubmed/8824514

M3 - Article

VL - 17

SP - 1919

EP - 1922

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 9

ER -

Hand PA, Inskip A, Gilford J, Alldersea J, Elexpuru-Camiruaga J, Hayes JD et al. Allelism at the glutathione S-transferase GSTM3 locus: interactions with GSTM1 and GSTT1 as risk factors for astrocytoma. Carcinogenesis. 1996 Sep;17(9):1919-22.