Abstract
Motivation: The current generation of DNA sequencing technologies produce a large amount of data quickly. All of these data need to pass some form of quality control (QC) processing and checking before they can be used for any analysis. The large number of samples that are run through Illumina sequencing machines makes the process of QC an onerous and time-consuming task that requires multiple pieces of information from several sources.
Results: AlmostSignificant is an open-source platform for aggregating multiple sources of quality metrics as well as run and sample meta-data associated with DNA sequencing runs from Illumina sequencing machines. AlmostSignificant is a graphical platform to streamline the QC of DNA sequencing data, to store these data for future reference together with extra meta-data associated with the sequencing runs not typically retained. This simplifies the challenge of monitoring the volume of data produced by Illumina sequencers. AlmostSignificant has been used to track the quality of over 80 sequencing runs covering over 2500 samples produced over the last three years.
Availability: The code and documentation for AlmostSignificant is freely available at https://github.com/bartongroup/AlmostSignificant CONTACT: c.cole@dundee.ac.uk, g.j.barton@dundee.ac.uk.
| Original language | English |
|---|---|
| Pages (from-to) | 3850-3851 |
| Number of pages | 2 |
| Journal | Bioinformatics |
| Volume | 32 |
| Issue number | 24 |
| Early online date | 24 Aug 2016 |
| DOIs | |
| Publication status | Published - 15 Dec 2016 |
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AlmostSignificant : simplifying quality control of high-throughput sequencing data. / Ward, Joseph; Cole, Christian (Lead / Corresponding author); Febrer, Melanie; Barton, Geoffrey J. (Lead / Corresponding author).
In: Bioinformatics, Vol. 32, No. 24, 15.12.2016, p. 3850-3851.Research output: Contribution to journal › Article
TY - JOUR
T1 - AlmostSignificant
T2 - simplifying quality control of high-throughput sequencing data
AU - Ward, Joseph
AU - Cole, Christian
AU - Febrer, Melanie
AU - Barton, Geoffrey J.
N1 - This work was supported by the Wellcome Trust Strategic Award 098439/Z/12/Z
PY - 2016/12/15
Y1 - 2016/12/15
N2 - Motivation: The current generation of DNA sequencing technologies produce a large amount of data quickly. All of these data need to pass some form of quality control (QC) processing and checking before they can be used for any analysis. The large number of samples that are run through Illumina sequencing machines makes the process of QC an onerous and time-consuming task that requires multiple pieces of information from several sources.Results: AlmostSignificant is an open-source platform for aggregating multiple sources of quality metrics as well as run and sample meta-data associated with DNA sequencing runs from Illumina sequencing machines. AlmostSignificant is a graphical platform to streamline the QC of DNA sequencing data, to store these data for future reference together with extra meta-data associated with the sequencing runs not typically retained. This simplifies the challenge of monitoring the volume of data produced by Illumina sequencers. AlmostSignificant has been used to track the quality of over 80 sequencing runs covering over 2500 samples produced over the last three years.Availability: The code and documentation for AlmostSignificant is freely available at https://github.com/bartongroup/AlmostSignificant CONTACT: c.cole@dundee.ac.uk, g.j.barton@dundee.ac.uk.
AB - Motivation: The current generation of DNA sequencing technologies produce a large amount of data quickly. All of these data need to pass some form of quality control (QC) processing and checking before they can be used for any analysis. The large number of samples that are run through Illumina sequencing machines makes the process of QC an onerous and time-consuming task that requires multiple pieces of information from several sources.Results: AlmostSignificant is an open-source platform for aggregating multiple sources of quality metrics as well as run and sample meta-data associated with DNA sequencing runs from Illumina sequencing machines. AlmostSignificant is a graphical platform to streamline the QC of DNA sequencing data, to store these data for future reference together with extra meta-data associated with the sequencing runs not typically retained. This simplifies the challenge of monitoring the volume of data produced by Illumina sequencers. AlmostSignificant has been used to track the quality of over 80 sequencing runs covering over 2500 samples produced over the last three years.Availability: The code and documentation for AlmostSignificant is freely available at https://github.com/bartongroup/AlmostSignificant CONTACT: c.cole@dundee.ac.uk, g.j.barton@dundee.ac.uk.
U2 - 10.1093/bioinformatics/btw559
DO - 10.1093/bioinformatics/btw559
M3 - Article
VL - 32
SP - 3850
EP - 3851
JO - Bioinformatics
JF - Bioinformatics
SN - 1367-4803
IS - 24
ER -