Altered aggregation properties of mutant γ-crystallins cause inherited cataract

Aileen Sandilands, Aileen M. Hutcheson, Heather A. Long, Alan R. Prescott, Gijs Vrensen, Jana Loster, Norman Klopp, Raimund B. Lutz, Jochen Graw, Shigeo Masaki, Christopher M. Dobson, Cait E. MacPhee, Roy A. Quinlan

    Research output: Contribution to journalArticlepeer-review

    119 Citations (Scopus)

    Abstract

    NOTE:THE SPECIAL CHARACTERS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE PUBLISHER'S WEBSITE FOR AN ACCURATE DISPLAY. Protein inclusions are associated with a diverse group of human diseases ranging from localized neurological disorders through to systemic non-neuropathic diseases. Here, we present evidence that the formation of intranuclear inclusions is a key event in cataract formation involving altered ?-crystallins that are unlikely to adopt their native fold. In three different inherited murine cataracts involving this type of ?-crystallin mutation, large inclusions containing the altered ?-crystallins were found in the nuclei of the primary lens fibre cells. Their formation preceded not only the first gross morphological changes in the lens, but also the first signs of cataract. The inclusions contained filamentous material that could be stained with the amyloid-detecting dye, Congo red. In vitro, recombinant mutant ?B-crystallin readily formed amyloid fibrils under physiological buffer conditions, unlike wild-type protein. These data suggest that this type of cataract is caused by a mechanism involving the nuclear targeting and deposition of amyloid-like inclusions. The mutant ?-crystallins initially disrupt nuclear function, but then this progresses to a full cataract phenotype.
    Original languageEnglish
    Pages (from-to)6005-6014
    Number of pages10
    JournalThe EMBO Journal
    Volume21
    Issue number22
    DOIs
    Publication statusPublished - Nov 2002

    Keywords

    • Amyloidosis
    • γ-crystallins
    • Protein misfolding

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