Altered signaling associated with chronic arsenic exposure in human skin keratinocytes

Sartaj Ahmad Mir, Santosh Renuse, Gajanan Sathe, Aafaque Ahmad Khan, Arun H. Patil, Vishalakshi Nanjappa, Firdous Ahmad Bhat, T. S.Keshava Prasad, Ashok K. Giri, Aditi Chatterjee, Harsha Gowda (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

Abstract

Modulation of signaling pathways upon chronic arsenic exposure remains poorly studied. Here, we carried out SILAC-based quantitative phosphoproteomics analysis to dissect the signaling induced upon chronic arsenic exposure in human skin keratinocyte cell line, HaCaT. We identified 4171 unique phosphosites derived from 2000 proteins. We observed differential phosphorylation of 406 phosphosites (twofold) corresponding to 305 proteins. Several pathways involved in cytoskeleton maintenance and organization were found to be significantly enriched (p<0.05). Our data revealed altered phosphorylation of proteins associated with adherens junction remodeling and actin polymerization. Kinases such as protein kinase C iota type (PRKCI), mitogen-activated protein kinase kinase kinase 1 (MAP3K1), tyrosine-protein kinase BAZ1B (BAZ1B) and STE20 like kinase (SLK) were found to be hyperphosphorylated. Our study provides novel insights into signaling perturbations associated with chronic arsenic exposure in human skin keratinocytes. All MS/MS data have been deposited to the ProteomeXchange with identifier PXD004868.

Original languageEnglish
Article number1700004
Number of pages5
JournalProteomics - Clinical Applications
Volume11
Issue number11-12
Early online date17 Aug 2017
DOIs
Publication statusPublished - 18 Dec 2017

Keywords

  • Arsenite
  • Mass spectrometry
  • Metabolic labeling
  • Phosphoproteome
  • Titanium-dioxide

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