Alternate thermoregulation and functional binding of Escherichia coli type 1 fimbriae in environmental and animal isolates

Jacqueline Marshall, Yannick Rossez, Geoffrey Mainda, David L. Gally, Tim J. Daniell, Nicola J. Holden

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Type 1 fimbriae (T1F) are well characterised cell surface organelles expressed by Escherichia coli and required for adherence to mannosylated host tissue. They satisfy molecular Koch's postulates as a virulence determinant and a host-adapted role has been reinforced by reports that T1F expression is repressed at submammalian temperatures. Analysis of a group of 136 environmental and animal E. coli isolates that express T1F at 37°C showed that 28% are also capable of expression at 20°C, in a phase variable manner. The heterogeneous proportions varied widely, and although growth temperature impacted the total proportion expressing T1F, there was no direct correlation between growth at 37°C and 20°C, indicative of differences in thermoregulation of the genetic switch (fimS) that controls phase variation. Specificities of the adhesin (FimH) also varied between the isolates: most bound to α-(1-3) mannan and yeast extracts as expected, but some recognised β-(1-4)-mannans and N-linked glycoproteins from plants, and T1F from two of the isolates mediated binding to plant roots. The results expand our view of a well-described adherence factor to show alternative expression profiles and adhesin specificities, which in turn may confer an advantage for certain isolates in alternative hosts and habitats.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalFEMS Microbiology Letters
Volume363
Issue number22
DOIs
Publication statusPublished - Nov 2016

Keywords

  • Adhesins, Escherichia coli
  • Bacterial adhesion
  • Cell extracts
  • Escherichia coli
  • Fimbriae proteins
  • Fimbriae, Bacterial
  • Glycoproteins
  • Mannans
  • Mannose
  • Plant roots
  • Protein binding
  • Temperature
  • Journal article

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