An acquired efflux system is responsible for copper resistance in Xanthomonas strain IG-8 isolated from China

Robert P. Ryan; David J. Ryan; Yi-Cheng Sun; Feng-Mei Li; Yiping Wang; David N. Dowling

doi:10.1111/j.1574-6968.2006.00592.x

An acquired efflux system is responsible for copper resistance in Xanthomonas strain IG-8 isolated from China

Robert P. Ryan, David J. Ryan, Yi-Cheng Sun, Feng-Mei Li, Yiping Wang, David N. Dowling

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Abstract

The genus Xanthomonas contains plant pathogens exhibiting innate resistance to a range of antimicrobial agents. In other genera, multidrug resistance is mediated by a synergy between a low-permeability outer membrane and expression of a number of multidrug efflux systems. This report describes the isolation of a novel gene cluster xmeRSA from Xanthomonas strain IG-8 that mediates copper chloride resistance. Subsequent analysis of these genes showed that they were responsible for the high level of multiple resistance in this strain and were homologues of the sme system of Stenotrophomonas maltophilia. Knock-out mutants of this gene cluster indicate that these genes are required for the copper resistance phenotype of strain IG-8. Expression analysis using lacZ fusions indicates that the genes are regulated by copper and other antimicrobials. Bioinformatic analysis suggests that these genes were acquired by horizontal gene transfer.

Original language	English
Pages (from-to)	40-46
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	268
Issue number	1
Early online date	11 Jan 2007
DOIs	https://doi.org/10.1111/j.1574-6968.2006.00592.x
Publication status	Published - 1 Mar 2007

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10.1111/j.1574-6968.2006.00592.x

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title = "An acquired efflux system is responsible for copper resistance in Xanthomonas strain IG-8 isolated from China",

abstract = "The genus Xanthomonas contains plant pathogens exhibiting innate resistance to a range of antimicrobial agents. In other genera, multidrug resistance is mediated by a synergy between a low-permeability outer membrane and expression of a number of multidrug efflux systems. This report describes the isolation of a novel gene cluster xmeRSA from Xanthomonas strain IG-8 that mediates copper chloride resistance. Subsequent analysis of these genes showed that they were responsible for the high level of multiple resistance in this strain and were homologues of the sme system of Stenotrophomonas maltophilia. Knock-out mutants of this gene cluster indicate that these genes are required for the copper resistance phenotype of strain IG-8. Expression analysis using lacZ fusions indicates that the genes are regulated by copper and other antimicrobials. Bioinformatic analysis suggests that these genes were acquired by horizontal gene transfer.",

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AU - Ryan, Robert P.

AU - Ryan, David J.

AU - Sun, Yi-Cheng

AU - Li, Feng-Mei

AU - Wang, Yiping

AU - Dowling, David N.

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AB - The genus Xanthomonas contains plant pathogens exhibiting innate resistance to a range of antimicrobial agents. In other genera, multidrug resistance is mediated by a synergy between a low-permeability outer membrane and expression of a number of multidrug efflux systems. This report describes the isolation of a novel gene cluster xmeRSA from Xanthomonas strain IG-8 that mediates copper chloride resistance. Subsequent analysis of these genes showed that they were responsible for the high level of multiple resistance in this strain and were homologues of the sme system of Stenotrophomonas maltophilia. Knock-out mutants of this gene cluster indicate that these genes are required for the copper resistance phenotype of strain IG-8. Expression analysis using lacZ fusions indicates that the genes are regulated by copper and other antimicrobials. Bioinformatic analysis suggests that these genes were acquired by horizontal gene transfer.

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An acquired efflux system is responsible for copper resistance in Xanthomonas strain IG-8 isolated from China

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