TY - JOUR
T1 - An Ana2/Ctp/Mud complex regulates spindle orientation in Drosophila neuroblasts
AU - Wang, Cheng
AU - Li, Song
AU - Januschke, Jens
AU - Rossi, Fabrizio
AU - Izumi, Yasushi
AU - Garcia-Alvarez, Gisela
AU - Gwee, Serene Sze Ling
AU - Soon, Swee Beng
AU - Sidhu, Harpreet Kaur
AU - Yu, Fengwei
AU - Matsuzaki, Fumio
AU - Gonzalez, Cayetano
AU - Wang, Hongyan
PY - 2011/9/13
Y1 - 2011/9/13
N2 - Drosophila neural stem cells, larval brain neuroblasts (NBs), align their mitotic spindles along the apical/basal axis during asymmetric cell division (ACD) to maintain the balance of self-renewal and differentiation. Here, we identified a protein complex composed of the tumor suppressor anastral spindle 2 (Ana2), a dynein light-chain protein Cut up (Ctp), and Mushroom body defect (Mud), which regulates mitotic spindle orientation. We isolated two ana2 alleles that displayed spindle misorientation and NB overgrowth phenotypes in larval brains. The centriolar protein Ana2 anchors Ctp to centrioles during ACD. The centriolar localization of Ctp is important for spindle orientation. Ana2 and Ctp localize Mud to the centrosomes and cell cortex and facilitate/maintain the association of Mud with Pins at the apical cortex. Our findings reveal that the centrosomal proteins Ana2 and Ctp regulate Mud function to orient the mitotic spindle during NB asymmetric division.
AB - Drosophila neural stem cells, larval brain neuroblasts (NBs), align their mitotic spindles along the apical/basal axis during asymmetric cell division (ACD) to maintain the balance of self-renewal and differentiation. Here, we identified a protein complex composed of the tumor suppressor anastral spindle 2 (Ana2), a dynein light-chain protein Cut up (Ctp), and Mushroom body defect (Mud), which regulates mitotic spindle orientation. We isolated two ana2 alleles that displayed spindle misorientation and NB overgrowth phenotypes in larval brains. The centriolar protein Ana2 anchors Ctp to centrioles during ACD. The centriolar localization of Ctp is important for spindle orientation. Ana2 and Ctp localize Mud to the centrosomes and cell cortex and facilitate/maintain the association of Mud with Pins at the apical cortex. Our findings reveal that the centrosomal proteins Ana2 and Ctp regulate Mud function to orient the mitotic spindle during NB asymmetric division.
U2 - 10.1016/j.devcel.2011.08.002
DO - 10.1016/j.devcel.2011.08.002
M3 - Article
C2 - 21920316
SN - 1878-1551
VL - 21
SP - 520
EP - 533
JO - Developmental Cell
JF - Developmental Cell
IS - 3
ER -