An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm

Miles R. Armstrong, Stephen C. Whisson, Leighton Pritchard, Jorunn I. B. Bos, Eduard Venter, Anna O. Avrova, Anne P. Rehmany, Ulrike Bohme, Karen Brooks, Inna Cherevach, Nancy Hamlin, Brian White, Audrey Fraser, Angela Lord, Michael A. Quail, Carol Churcher, Neil Hall, Matthew Berriman, Sanwen Huang, Sophien KamounJim L. Beynon, Paul R. J. Birch, R. James Cook

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    312 Citations (Scopus)

    Abstract

    NOTE: THE SPECIAL CHARACTERS IN THIS ABSTRACT CANNOT BE DISPLAYED CORRECTLY ON THIS PAGE. PLEASE REFER TO THE ABSTRACT ON THE PUBLISHER'S WEBSITE FOR AN ACCURATE DISPLAY. The oomycete Phytophthora infestans causes late blight, the potato disease that precipitated the Irish famines in 1846 and 1847. It represents a reemerging threat to potato production and is one of >70 species that are arguably the most devastating pathogens of dicotyledonous plants. Nevertheless, little is known about the molecular bases of pathogenicity in these algae-like organisms or of avirulence molecules that are perceived by host defenses. Disease resistance alleles, products of which recognize corresponding avirulence molecules in the pathogen, have been introgressed into the cultivated potato from a wild species, Solanum demissum, and R1 and R3a have been identified. We used association genetics to identify Avr3a and show that it encodes a protein that is recognized in the host cytoplasm, where it triggers R3a-dependent cell death. Avr3a resides in a region of the P. infestans genome that is colinear with the locus containing avirulence gene ATR1NdWsB in Hyaloperonospora parasitica, an oomycete pathogen of Arabidopsis. Remarkably, distances between conserved genes in these avirulence loci were often similar, despite intervening genomic variation. We suggest that Avr3a has undergone gene duplication and that an allele evading recognition by R3a arose under positive selection.
    Original languageEnglish
    Pages (from-to)7766-7771
    Number of pages6
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume102
    Issue number21
    DOIs
    Publication statusPublished - May 2005

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    Keywords

    • Microsynteny
    • Phytophthora infestans
    • Hypersensitive response
    • Linkage disequilibrium
    • Hemibiotroph

    Cite this

    Armstrong, M. R., Whisson, S. C., Pritchard, L., Bos, J. I. B., Venter, E., Avrova, A. O., Rehmany, A. P., Bohme, U., Brooks, K., Cherevach, I., Hamlin, N., White, B., Fraser, A., Lord, A., Quail, M. A., Churcher, C., Hall, N., Berriman, M., Huang, S., ... Cook, R. J. (Ed.) (2005). An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm. Proceedings of the National Academy of Sciences of the United States of America, 102(21), 7766-7771. https://doi.org/10.1073/pnas.0500113102