An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment

Carl A. Webster; Desire Di Silvio; Aarthi Devarajan; Paolo Bigini; Edoardo Micotti; Chiara Giudice; Mario Salmona; Grant N. Wheeler; Victoria Sherwood; Francesca Baldelli Bombelli

doi:10.2217/nnm.15.219

An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment

Carl A. Webster, Desire Di Silvio, Aarthi Devarajan, Paolo Bigini, Edoardo Micotti, Chiara Giudice, Mario Salmona, Grant N. Wheeler, Victoria Sherwood (Lead / Corresponding author), Francesca Baldelli Bombelli (Lead / Corresponding author)

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Abstract

Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.

Original language	English
Pages (from-to)	643-656
Number of pages	14
Journal	Nanomedicine
Volume	11
Issue number	6
DOIs	https://doi.org/10.2217/nnm.15.219
Publication status	Published - Mar 2016

Keywords

cytotoxicity
in vitro-in vivo correlations
nanomaterials
nanomedicine
nanotoxicity
Xenopus laevis

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Webster, C. A., Di Silvio, D., Devarajan, A., Bigini, P., Micotti, E., Giudice, C., Salmona, M., Wheeler, G. N., Sherwood, V., & Bombelli, F. B. (2016). An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment. Nanomedicine, 11(6), 643-656. https://doi.org/10.2217/nnm.15.219

Webster, Carl A. ; Di Silvio, Desire ; Devarajan, Aarthi ; Bigini, Paolo ; Micotti, Edoardo ; Giudice, Chiara ; Salmona, Mario ; Wheeler, Grant N. ; Sherwood, Victoria ; Bombelli, Francesca Baldelli. / An early developmental vertebrate model for nanomaterial safety : Bridging cell-based and mammalian toxicity assessment. In: Nanomedicine. 2016 ; Vol. 11, No. 6. pp. 643-656.

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abstract = "Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.",

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note = "This work was supported by funds from The British Skin Foundation, The Royal Society and UEA start up funds to V Sherwood and FB Bombelli. FB Bombelli would like to thank for additional financial support RegioneLombardia (Fondo per lo Sviluppo e la Coesione - FAS 2007–2013). V Sherwood is supported by a CRUK programme grant awarded to the CRUKSkin Tumour Laboratory, Medical School, University of Dundee. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed",

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An early developmental vertebrate model for nanomaterial safety : Bridging cell-based and mammalian toxicity assessment. / Webster, Carl A.; Di Silvio, Desire; Devarajan, Aarthi; Bigini, Paolo; Micotti, Edoardo; Giudice, Chiara; Salmona, Mario; Wheeler, Grant N.; Sherwood, Victoria (Lead / Corresponding author); Bombelli, Francesca Baldelli (Lead / Corresponding author).

In: Nanomedicine, Vol. 11, No. 6, 03.2016, p. 643-656.

Research output: Contribution to journal › Article

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AU - Wheeler, Grant N.

AU - Sherwood, Victoria

AU - Bombelli, Francesca Baldelli

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