An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment

Carl A. Webster; Desire Di Silvio; Aarthi Devarajan; Paolo Bigini; Edoardo Micotti; Chiara Giudice; Mario Salmona; Grant N. Wheeler; Victoria Sherwood; Francesca Baldelli Bombelli

doi:10.2217/nnm.15.219

An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment

Carl A. Webster, Desire Di Silvio, Aarthi Devarajan, Paolo Bigini, Edoardo Micotti, Chiara Giudice, Mario Salmona, Grant N. Wheeler, Victoria Sherwood (Lead / Corresponding author), Francesca Baldelli Bombelli (Lead / Corresponding author)

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Abstract

Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.

Original language	English
Pages (from-to)	643-656
Number of pages	14
Journal	Nanomedicine
Volume	11
Issue number	6
DOIs	https://doi.org/10.2217/nnm.15.219
Publication status	Published - Mar 2016

Keywords

cytotoxicity
in vitro-in vivo correlations
nanomaterials
nanomedicine
nanotoxicity
Xenopus laevis

ASJC Scopus subject areas

Materials Science(all)
Bioengineering
Biomedical Engineering
Medicine (miscellaneous)
Development

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10.2217/nnm.15.219Licence: CC BY-NC

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Final published version, 3.58 MBLicence: CC BY-NC

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title = "An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment",

abstract = "Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.",

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author = "Webster, {Carl A.} and {Di Silvio}, Desire and Aarthi Devarajan and Paolo Bigini and Edoardo Micotti and Chiara Giudice and Mario Salmona and Wheeler, {Grant N.} and Victoria Sherwood and Bombelli, {Francesca Baldelli}",

note = "This work was supported by funds from The British Skin Foundation, The Royal Society and UEA start up funds to V Sherwood and FB Bombelli. FB Bombelli would like to thank for additional financial support RegioneLombardia (Fondo per lo Sviluppo e la Coesione - FAS 2007–2013). V Sherwood is supported by a CRUK programme grant awarded to the CRUKSkin Tumour Laboratory, Medical School, University of Dundee. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed",

year = "2016",

month = mar,

doi = "10.2217/nnm.15.219",

language = "English",

volume = "11",

pages = "643--656",

journal = "Nanomedicine",

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T1 - An early developmental vertebrate model for nanomaterial safety

T2 - Bridging cell-based and mammalian toxicity assessment

AU - Webster, Carl A.

AU - Di Silvio, Desire

AU - Devarajan, Aarthi

AU - Bigini, Paolo

AU - Micotti, Edoardo

AU - Giudice, Chiara

AU - Salmona, Mario

AU - Wheeler, Grant N.

AU - Sherwood, Victoria

AU - Bombelli, Francesca Baldelli

N1 - This work was supported by funds from The British Skin Foundation, The Royal Society and UEA start up funds to V Sherwood and FB Bombelli. FB Bombelli would like to thank for additional financial support RegioneLombardia (Fondo per lo Sviluppo e la Coesione - FAS 2007–2013). V Sherwood is supported by a CRUK programme grant awarded to the CRUKSkin Tumour Laboratory, Medical School, University of Dundee. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed

PY - 2016/3

Y1 - 2016/3

N2 - Aim: With the rise in production of nanoparticles (NPs) for an ever-increasing number of applications, there is an urgent need to efficiently assess their potential toxicity. We propose a NP hazard assessment protocol that combines mammalian cytotoxicity data with embryonic vertebrate abnormality scoring to determine an overall toxicity index. Results: We observed that, after exposure to a range of NPs, Xenopus phenotypic scoring showed a strong correlation with cell based in vitro assays. Magnetite-cored NPs, negative for toxicity in vitro and Xenopus, were further confirmed as nontoxic in mice. Conclusion: The results highlight the potential of Xenopus embryo analysis as a fast screening approach for toxicity assessment of NPs, which could be introduced for the routine testing of nanomaterials.

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An early developmental vertebrate model for nanomaterial safety: Bridging cell-based and mammalian toxicity assessment

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Keywords

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