An electrophysiological investigation of the characteristics and function of GABAA receptors on bovine adrenomedullary chromaffin cells

J. A. Peters, J. J. Lambert, G. A. Cottrell

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Abstract

The characteristics and function of γ-aminobutyric acidA (GABAA) receptors expressed on bovine chromaffin cells in culture have been investigated using patch-clamp techniques. In voltage-clamped whole-cells, locally applied GABA (100 μM) evoked a transmembrane chloride current which demonstrated outward rectification. The amplitude of such currents was reversibly suppressed by the GABAA receptor antagonists bicuculline, picrotoxin and RU5135, and enhanced by the general anaesthetic propanidid. Glycine (100 μM) and baclofen (100 μM) were ineffective as agonists. In support of a physiological role for GABA in the adrenal medulla, the co-existence of GABAA and nicotinic acetylcholine (ACh) receptors was demonstrated on whole cells and outside-out membrane patches. Ionophoretically applied GABA reduced the amplitude of depolarization and action potential discharge occurring in response to locally applied ACh (100 μM), but had no effect upon the underlying ACh-induced current. In addition, an excitatory action of GABA was demonstrated by recording action potential waveforms in cell-attached patches. The results are discussed in the context of a GABA-ergic regulation of catecholamine secretion.

Original languageEnglish
Pages (from-to)95-103
Number of pages9
JournalPflügers Archiv European Journal of Physiology
Volume415
Issue number1
DOIs
Publication statusPublished - Oct 1989

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Chromaffin Cells
GABA-A Receptors
gamma-Aminobutyric Acid
Action Potentials
Acetylcholine
Propanidid
GABA-A Receptor Antagonists
Picrotoxin
General Anesthetics
Baclofen
Adrenal Medulla
Bicuculline
Induced currents
Depolarization
Clamping devices
Nicotinic Receptors
Patch-Clamp Techniques
Glycine
Catecholamines
Chlorides

Keywords

  • Adrenal medulla
  • Bovine chromaffin cells
  • Catecholamine secretion
  • GABA receptors
  • Nicotinic receptors
  • Patch-clamp technique

Cite this

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title = "An electrophysiological investigation of the characteristics and function of GABAA receptors on bovine adrenomedullary chromaffin cells",
abstract = "The characteristics and function of γ-aminobutyric acidA (GABAA) receptors expressed on bovine chromaffin cells in culture have been investigated using patch-clamp techniques. In voltage-clamped whole-cells, locally applied GABA (100 μM) evoked a transmembrane chloride current which demonstrated outward rectification. The amplitude of such currents was reversibly suppressed by the GABAA receptor antagonists bicuculline, picrotoxin and RU5135, and enhanced by the general anaesthetic propanidid. Glycine (100 μM) and baclofen (100 μM) were ineffective as agonists. In support of a physiological role for GABA in the adrenal medulla, the co-existence of GABAA and nicotinic acetylcholine (ACh) receptors was demonstrated on whole cells and outside-out membrane patches. Ionophoretically applied GABA reduced the amplitude of depolarization and action potential discharge occurring in response to locally applied ACh (100 μM), but had no effect upon the underlying ACh-induced current. In addition, an excitatory action of GABA was demonstrated by recording action potential waveforms in cell-attached patches. The results are discussed in the context of a GABA-ergic regulation of catecholamine secretion.",
keywords = "Adrenal medulla, Bovine chromaffin cells, Catecholamine secretion, GABA receptors, Nicotinic receptors, Patch-clamp technique",
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AU - Peters, J. A.

AU - Lambert, J. J.

AU - Cottrell, G. A.

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N2 - The characteristics and function of γ-aminobutyric acidA (GABAA) receptors expressed on bovine chromaffin cells in culture have been investigated using patch-clamp techniques. In voltage-clamped whole-cells, locally applied GABA (100 μM) evoked a transmembrane chloride current which demonstrated outward rectification. The amplitude of such currents was reversibly suppressed by the GABAA receptor antagonists bicuculline, picrotoxin and RU5135, and enhanced by the general anaesthetic propanidid. Glycine (100 μM) and baclofen (100 μM) were ineffective as agonists. In support of a physiological role for GABA in the adrenal medulla, the co-existence of GABAA and nicotinic acetylcholine (ACh) receptors was demonstrated on whole cells and outside-out membrane patches. Ionophoretically applied GABA reduced the amplitude of depolarization and action potential discharge occurring in response to locally applied ACh (100 μM), but had no effect upon the underlying ACh-induced current. In addition, an excitatory action of GABA was demonstrated by recording action potential waveforms in cell-attached patches. The results are discussed in the context of a GABA-ergic regulation of catecholamine secretion.

AB - The characteristics and function of γ-aminobutyric acidA (GABAA) receptors expressed on bovine chromaffin cells in culture have been investigated using patch-clamp techniques. In voltage-clamped whole-cells, locally applied GABA (100 μM) evoked a transmembrane chloride current which demonstrated outward rectification. The amplitude of such currents was reversibly suppressed by the GABAA receptor antagonists bicuculline, picrotoxin and RU5135, and enhanced by the general anaesthetic propanidid. Glycine (100 μM) and baclofen (100 μM) were ineffective as agonists. In support of a physiological role for GABA in the adrenal medulla, the co-existence of GABAA and nicotinic acetylcholine (ACh) receptors was demonstrated on whole cells and outside-out membrane patches. Ionophoretically applied GABA reduced the amplitude of depolarization and action potential discharge occurring in response to locally applied ACh (100 μM), but had no effect upon the underlying ACh-induced current. In addition, an excitatory action of GABA was demonstrated by recording action potential waveforms in cell-attached patches. The results are discussed in the context of a GABA-ergic regulation of catecholamine secretion.

KW - Adrenal medulla

KW - Bovine chromaffin cells

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KW - Nicotinic receptors

KW - Patch-clamp technique

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