An essential role for calmodulin in regulating human T cell aggregation

Susanna C. Fagerholm; Alan Prescott; Philip Cohen; Carl G. Gahmberg; Veli-Pekka Lehto

doi:10.1016/S0014-5793(01)02182-2

An essential role for calmodulin in regulating human T cell aggregation

Susanna C. Fagerholm
, Alan Prescott
, Philip Cohen
, Carl G. Gahmberg
, Veli-Pekka Lehto (Editor)

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

After activation of T cells with either CD3 antibodies or phorbol esters, we have found that T cell-cell aggregation, integrin-dependent actin reorganisation and cell spreading are strongly suppressed by any of three structurally different calmodulin antagonists, without any effect on the amount of CD11/CD18 integrin binding to the actin cytoskeleton. However, only T cell receptor-induced, and not phorbol ester-induced, aggregation and cell spreading are prevented by inhibitors of phosphatidylinositide (PI) 3-kinase. These results suggest that PI 3-kinase lies upstream of calmodulin in the signalling pathway leading to T cell aggregation, cell spreading and actin reorganisation and that cell spreading and actin reorganisation are essential for T cell adhesion.

Original language	English
Pages (from-to)	131-6
Number of pages	6
Journal	FEBS Letters
Volume	491
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(01)02182-2
Publication status	Published - 28 Feb 2001

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title = "An essential role for calmodulin in regulating human T cell aggregation",

abstract = "After activation of T cells with either CD3 antibodies or phorbol esters, we have found that T cell-cell aggregation, integrin-dependent actin reorganisation and cell spreading are strongly suppressed by any of three structurally different calmodulin antagonists, without any effect on the amount of CD11/CD18 integrin binding to the actin cytoskeleton. However, only T cell receptor-induced, and not phorbol ester-induced, aggregation and cell spreading are prevented by inhibitors of phosphatidylinositide (PI) 3-kinase. These results suggest that PI 3-kinase lies upstream of calmodulin in the signalling pathway leading to T cell aggregation, cell spreading and actin reorganisation and that cell spreading and actin reorganisation are essential for T cell adhesion.",

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AU - Fagerholm, Susanna C.

AU - Prescott, Alan

AU - Cohen, Philip

AU - Gahmberg, Carl G.

A2 - Lehto, Veli-Pekka

PY - 2001/2/28

Y1 - 2001/2/28

N2 - After activation of T cells with either CD3 antibodies or phorbol esters, we have found that T cell-cell aggregation, integrin-dependent actin reorganisation and cell spreading are strongly suppressed by any of three structurally different calmodulin antagonists, without any effect on the amount of CD11/CD18 integrin binding to the actin cytoskeleton. However, only T cell receptor-induced, and not phorbol ester-induced, aggregation and cell spreading are prevented by inhibitors of phosphatidylinositide (PI) 3-kinase. These results suggest that PI 3-kinase lies upstream of calmodulin in the signalling pathway leading to T cell aggregation, cell spreading and actin reorganisation and that cell spreading and actin reorganisation are essential for T cell adhesion.

AB - After activation of T cells with either CD3 antibodies or phorbol esters, we have found that T cell-cell aggregation, integrin-dependent actin reorganisation and cell spreading are strongly suppressed by any of three structurally different calmodulin antagonists, without any effect on the amount of CD11/CD18 integrin binding to the actin cytoskeleton. However, only T cell receptor-induced, and not phorbol ester-induced, aggregation and cell spreading are prevented by inhibitors of phosphatidylinositide (PI) 3-kinase. These results suggest that PI 3-kinase lies upstream of calmodulin in the signalling pathway leading to T cell aggregation, cell spreading and actin reorganisation and that cell spreading and actin reorganisation are essential for T cell adhesion.

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DO - 10.1016/S0014-5793(01)02182-2

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VL - 491

SP - 131

EP - 136

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

An essential role for calmodulin in regulating human T cell aggregation

Abstract

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