An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley

Hui Liu, Micha Bayer, Arnis Druka, Joanne R. Russell, Christine A. Hackett, Jesse Poland, Luke Ramsay, Pete E. Hedley, Robbie Waugh

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    Abstract

    We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar 'Golden Promise' (ari-e.GP/Vrs1) and the six-rowed cultivar 'Morex' (Ari-e/vrs1) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene Vrs1 on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene Breviaristatum-e (Ari-e).

    Original languageEnglish
    Article number104
    Number of pages11
    JournalBMC Genomics
    Volume15
    DOIs
    Publication statusPublished - 6 Feb 2014

    Keywords

    • Alleles
    • Chromosome Mapping
    • Genes, Plant
    • Genetic Linkage
    • Genotype
    • Hordeum
    • Phenotype
    • Polymorphism, Single Nucleotide
    • Quantitative Trait Loci
    • Sequence Analysis, DNA

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