An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley

Hui Liu; Micha Bayer; Arnis Druka; Joanne R. Russell; Christine A. Hackett; Jesse Poland; Luke Ramsay; Pete E. Hedley; Robbie Waugh

doi:10.1186/1471-2164-15-104

An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley

Hui Liu
, Micha Bayer
, Arnis Druka
, Joanne R. Russell
, Christine A. Hackett
, Jesse Poland
, Luke Ramsay
, Pete E. Hedley
, Robbie Waugh

Research output: Contribution to journal › Article › peer-review

123 Citations (Scopus)

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Abstract

We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar 'Golden Promise' (ari-e.GP/Vrs1) and the six-rowed cultivar 'Morex' (Ari-e/vrs1) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene Vrs1 on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene Breviaristatum-e (Ari-e).

Original language	English
Article number	104
Number of pages	11
Journal	BMC Genomics
Volume	15
DOIs	https://doi.org/10.1186/1471-2164-15-104
Publication status	Published - 6 Feb 2014

Keywords

Alleles
Chromosome Mapping
Genes, Plant
Genetic Linkage
Genotype
Hordeum
Phenotype
Polymorphism, Single Nucleotide
Quantitative Trait Loci
Sequence Analysis, DNA

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10.1186/1471-2164-15-104

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Final published version, 268 KBLicence: CC BY

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Cite this

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title = "An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley",

abstract = "We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar 'Golden Promise' (ari-e.GP/Vrs1) and the six-rowed cultivar 'Morex' (Ari-e/vrs1) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene Vrs1 on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene Breviaristatum-e (Ari-e).",

keywords = "Alleles, Chromosome Mapping, Genes, Plant, Genetic Linkage, Genotype, Hordeum, Phenotype, Polymorphism, Single Nucleotide, Quantitative Trait Loci, Sequence Analysis, DNA",

author = "Hui Liu and Micha Bayer and Arnis Druka and Russell, \{Joanne R.\} and Hackett, \{Christine A.\} and Jesse Poland and Luke Ramsay and Hedley, \{Pete E.\} and Robbie Waugh",

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doi = "10.1186/1471-2164-15-104",

language = "English",

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T1 - An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley

AU - Liu, Hui

AU - Bayer, Micha

AU - Druka, Arnis

AU - Russell, Joanne R.

AU - Hackett, Christine A.

AU - Poland, Jesse

AU - Ramsay, Luke

AU - Hedley, Pete E.

AU - Waugh, Robbie

PY - 2014/2/6

Y1 - 2014/2/6

N2 - We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar 'Golden Promise' (ari-e.GP/Vrs1) and the six-rowed cultivar 'Morex' (Ari-e/vrs1) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene Vrs1 on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene Breviaristatum-e (Ari-e).

AB - We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar 'Golden Promise' (ari-e.GP/Vrs1) and the six-rowed cultivar 'Morex' (Ari-e/vrs1) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene Vrs1 on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene Breviaristatum-e (Ari-e).

KW - Alleles

KW - Chromosome Mapping

KW - Genes, Plant

KW - Genetic Linkage

KW - Genotype

KW - Hordeum

KW - Phenotype

KW - Polymorphism, Single Nucleotide

KW - Quantitative Trait Loci

KW - Sequence Analysis, DNA

U2 - 10.1186/1471-2164-15-104

DO - 10.1186/1471-2164-15-104

M3 - Article

C2 - 24498911

SN - 1471-2164

VL - 15

JO - BMC Genomics

JF - BMC Genomics

M1 - 104

ER -

An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley

Abstract

Keywords

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