An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues

Philip Cohen, Susanne Klumpp, Donald L. Schelling

    Research output: Contribution to journalArticle

    412 Citations (Scopus)

    Abstract

    The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1-2 nM okadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 μM okadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.

    Original languageEnglish
    Pages (from-to)596-600
    Number of pages5
    JournalFEBS Letters
    Volume250
    Issue number2
    DOIs
    Publication statusPublished - 3 Jul 1989

    Keywords

    • Inhibitor protein
    • Okadaic acid
    • Protein phosphatase
    • Protein phosphorylation
    • Tumor promoter

    Fingerprint Dive into the research topics of 'An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues'. Together they form a unique fingerprint.

  • Cite this