Abstract
The type 2A protein phosphatases in mammalian tissue extracts are inhibited completely and specifically by 1-2 nM okadaic acid. In contrast, type 1 protein phosphatases are hardly affected at these concentrations, complete inhibition requiring 1 μM okadaic acid. These observations have been exploited to develop an improved procedure for the identification and quantitation of type 1, type 2A and type 2C protein phosphatases in tissue extracts.
Original language | English |
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Pages (from-to) | 596-600 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 250 |
Issue number | 2 |
DOIs | |
Publication status | Published - 3 Jul 1989 |
Keywords
- Inhibitor protein
- Okadaic acid
- Protein phosphatase
- Protein phosphorylation
- Tumor promoter
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology