An improved rapid method for DNA recovery from cotton swabs

Alexander Gray; Agnieszka Kuffel; Niamh Nic Daeid

doi:10.1016/j.fsigen.2023.102848

An improved rapid method for DNA recovery from cotton swabs

Alexander Gray (Lead / Corresponding author), Agnieszka Kuffel, Niamh Nic Daeid

Leverhulme Research Centre for Forensic Science

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Abstract

We present a novel rapid method for the recovery of cellular and free DNA from cotton swabs based on a simple elution buffer containing a high molecular weight polymer and detergent combined with a short proteinase K digestion to release cellular DNA. This method shows increased yields approaching 80% recovery of the input DNA compared to the QIAamp DNA Mini kit standard extraction protocol for swabs which has a recovery of 20–30%. The buffer components in the described method are compatible with direct PCR analysis of the isolated DNA without further purification. Recovery efficiencies were estimated by qPCR.

Original language	English
Article number	102848
Number of pages	11
Journal	Forensic Science International: Genetics
Volume	64
Early online date	16 Feb 2023
DOIs	https://doi.org/10.1016/j.fsigen.2023.102848
Publication status	E-pub ahead of print - 16 Feb 2023

Keywords

Cotton swabs
Direct PCR
DNA recovery
Extraction

ASJC Scopus subject areas

Pathology and Forensic Medicine
Genetics

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10.1016/j.fsigen.2023.102848Licence: CC BY-NC-ND

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title = "An improved rapid method for DNA recovery from cotton swabs",

abstract = "We present a novel rapid method for the recovery of cellular and free DNA from cotton swabs based on a simple elution buffer containing a high molecular weight polymer and detergent combined with a short proteinase K digestion to release cellular DNA. This method shows increased yields approaching 80% recovery of the input DNA compared to the QIAamp DNA Mini kit standard extraction protocol for swabs which has a recovery of 20–30%. The buffer components in the described method are compatible with direct PCR analysis of the isolated DNA without further purification. Recovery efficiencies were estimated by qPCR.",

keywords = "Cotton swabs, Direct PCR, DNA recovery, Extraction",

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note = "Funding Information: This work has been funded by Leverhulme Trust grant. RC-1015-01 . We would also like to acknowledge the kind contribution of tissue culture cells from Dr Simon Hawley, University of Dundee School of Life Sciences and the gift of Investigator Go kits from Qiagen Ltd. Copyright: {\textcopyright} 2023 The Authors",

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N2 - We present a novel rapid method for the recovery of cellular and free DNA from cotton swabs based on a simple elution buffer containing a high molecular weight polymer and detergent combined with a short proteinase K digestion to release cellular DNA. This method shows increased yields approaching 80% recovery of the input DNA compared to the QIAamp DNA Mini kit standard extraction protocol for swabs which has a recovery of 20–30%. The buffer components in the described method are compatible with direct PCR analysis of the isolated DNA without further purification. Recovery efficiencies were estimated by qPCR.

AB - We present a novel rapid method for the recovery of cellular and free DNA from cotton swabs based on a simple elution buffer containing a high molecular weight polymer and detergent combined with a short proteinase K digestion to release cellular DNA. This method shows increased yields approaching 80% recovery of the input DNA compared to the QIAamp DNA Mini kit standard extraction protocol for swabs which has a recovery of 20–30%. The buffer components in the described method are compatible with direct PCR analysis of the isolated DNA without further purification. Recovery efficiencies were estimated by qPCR.

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An improved rapid method for DNA recovery from cotton swabs

Abstract

Keywords

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