An improved rapid method for DNA recovery from cotton swabs

Alexander Gray (Lead / Corresponding author), Agnieszka Kuffel, Niamh Nic Daeid

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We present a novel rapid method for the recovery of cellular and free DNA from cotton swabs based on a simple elution buffer containing a high molecular weight polymer and detergent combined with a short proteinase K digestion to release cellular DNA. This method shows increased yields approaching 80% recovery of the input DNA compared to the QIAamp DNA Mini kit standard extraction protocol for swabs which has a recovery of 20–30%. The buffer components in the described method are compatible with direct PCR analysis of the isolated DNA without further purification. Recovery efficiencies were estimated by qPCR.

Original languageEnglish
Article number102848
Number of pages11
JournalForensic Science International: Genetics
Early online date16 Feb 2023
Publication statusE-pub ahead of print - 16 Feb 2023


  • Cotton swabs
  • Direct PCR
  • DNA recovery
  • Extraction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Genetics


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