An in situ study investigating dentine tubule occlusion of dentifrices following acid challenge

Ryan C Olley, Peter Pilecki, Nathan Hughes, Peter Jeffery, Rupert S Austin, Rebecca Moazzez, David Bartlett

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)


OBJECTIVES: To investigate the dentine occlusion and acid resistance of dentifrices developed to treat dentine hypersensitivity.

METHODS: This was a single centre, single blind, randomised, split mouth, four treatments, two period crossover, in situ study in healthy subjects. Subjects wore buccal intra-oral appliances each fitted with four dentine samples over four consecutive days with one study product applied per appliance; 8% strontium acetate in silica base, 1040 ppm sodium fluoride (Sensodyne(®) Rapid Relief), 8% arginine, calcium carbonate, 1450 ppm sodium monofluorophosphate (Colgate Sensitive Pro-Relief(®)), 1450 ppm sodium fluoride (control paste) and water. On days 3 and 4, two agitated grapefruit juice challenges (ex vivo) occurred for 1 min. At the end of each treatment day 1 dentine sample was removed from each appliance for scanning electron microscopy (SEM). The extent of tubule occlusion was measured using an examiner-based visual scoring index (three trained examiners).

RESULTS: In total, 28 subjects ((12 males and 16 females with a mean age of 34.7 years (SD 8.41 years)) completed the study. On day 2, both test dentifrices demonstrated significantly better dentine tubule occlusion than water (p < 0.0001) and control paste (8% strontium p = 0.0003 and 8% arginine p = 0.0019). After 3 and 4 days of twice daily brushing with acid challenges on days 3 and 4 the strontium-based dentifrice demonstrated significantly better dentine occlusion than all other treatments (p < 0.0001).

CONCLUSIONS: Strontium acetate and arginine-based dentifrice result in statistically significant dentine tubular occlusion compared to controls, but the arginine-based dentifrice is more susceptible to acid challenge.

CLINICAL SIGNIFICANCE: Erosive beverages are an important aetiology in DH by exposing dentine tubules. Their consumption has increased significantly over the past decade in the UK. This 4-day in situ study investigated the properties of commercially available dentifrices designed to occlude dentine tubules and their resistance to an agitated acid challenge.

Original languageEnglish
Pages (from-to)585-93
Number of pages9
JournalJournal of Dentistry
Issue number7
Publication statusPublished - Jul 2012


  • Acetates
  • Adult
  • Arginine
  • Beverages
  • Calcium Carbonate
  • Carbonated Beverages
  • Carbonic Acid
  • Citric Acid
  • Citrus paradisi
  • Citrus sinensis
  • Cross-Over Studies
  • Dentifrices
  • Dentin
  • Dentin Desensitizing Agents
  • Female
  • Fluorides
  • Humans
  • Male
  • Microscopy, Electron, Scanning
  • Phosphates
  • Single-Blind Method
  • Sodium Fluoride
  • Strontium
  • Comparative Study
  • Journal Article
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't


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