An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery

F. Stewart; B. F. Cox; G. Wang; Z. Huang; I. P. Newton; I. S. Nathke; M. Thanou; S. Cochran

doi:10.1109/ULTSYM.2016.7728701

An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery

F. Stewart, B. F. Cox, G. Wang, Z. Huang, I. P. Newton, I. S. Nathke, M. Thanou, S. Cochran

School of Life Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Citations (Scopus)

Abstract

Ultrasound is a well-established imaging modality used in clinics worldwide for many different applications. It has also experienced recent growth in interest for therapeutic applications in the form of high-intensity focused ultrasound (HIFU) or focused ultrasound (focused-US), including in capsules for use in drug delivery in the gut. The work described here aimed to support the investigation of its usefulness for drug delivery further. An in vitro sonication system was designed, comprising a focused-US transducer, a cell plate on a vertical stage and transepithelial electrical resistance (TER) meter. Miniature focused-US transducers were fabricated with PZ26 and PZ54 materials (Meggitt Sensing Systems, Kvistgaard, Denmark). The active elements in both transducers have outer diameter, OD = 5 mm, and radius of curvature, R_C = 15 mm. US field mapping indicated that output acoustic pressures are in the range 25 kPa <P_ac <210 kPa and acoustic powers in the range 8 mW <Power_ac <80 mW. Feasibility testing with Caco-2 colon cancer cells involved measuring TER during and after sonication. Sonication produced a drop in TER of 6.7% after 6 min. Recovery to the starting TER value was observed 14 min after sonication indicating that the sonication system can be used to measure the effect of ultrasound on tissue barrier function as expected.

Original language	English
Title of host publication	2016 IEEE International Ultrasonics Symposium IUS (2016)
Publisher	IEEE Computer Society
Pages	1-4
Number of pages	4
Volume	2016-November
ISBN (Electronic)	9781467398978
DOIs	https://doi.org/10.1109/ULTSYM.2016.7728701
Publication status	Published - 3 Nov 2016
Event	2016 IEEE International Ultrasonics Symposium, IUS 2016 - Tours, France Duration: 18 Sept 2016 → 21 Sept 2016

Conference

Conference	2016 IEEE International Ultrasonics Symposium, IUS 2016
Country/Territory	France
City	Tours
Period	18/09/16 → 21/09/16

Keywords

cell sonication
focused ultrasound
focused ultrasound transducer
in vitro sonication
sonicator

ASJC Scopus subject areas

Acoustics and Ultrasonics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1109/ULTSYM.2016.7728701

Cite this

Stewart, F., Cox, B. F., Wang, G., Huang, Z., Newton, I. P., Nathke, I. S., Thanou, M., & Cochran, S. (2016). An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery. In 2016 IEEE International Ultrasonics Symposium IUS (2016) (Vol. 2016-November, pp. 1-4). Article 7728701 IEEE Computer Society. https://doi.org/10.1109/ULTSYM.2016.7728701

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title = "An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery",

abstract = "Ultrasound is a well-established imaging modality used in clinics worldwide for many different applications. It has also experienced recent growth in interest for therapeutic applications in the form of high-intensity focused ultrasound (HIFU) or focused ultrasound (focused-US), including in capsules for use in drug delivery in the gut. The work described here aimed to support the investigation of its usefulness for drug delivery further. An in vitro sonication system was designed, comprising a focused-US transducer, a cell plate on a vertical stage and transepithelial electrical resistance (TER) meter. Miniature focused-US transducers were fabricated with PZ26 and PZ54 materials (Meggitt Sensing Systems, Kvistgaard, Denmark). The active elements in both transducers have outer diameter, OD = 5 mm, and radius of curvature, RC = 15 mm. US field mapping indicated that output acoustic pressures are in the range 25 kPa <Pac <210 kPa and acoustic powers in the range 8 mW <Powerac <80 mW. Feasibility testing with Caco-2 colon cancer cells involved measuring TER during and after sonication. Sonication produced a drop in TER of 6.7% after 6 min. Recovery to the starting TER value was observed 14 min after sonication indicating that the sonication system can be used to measure the effect of ultrasound on tissue barrier function as expected.",

keywords = "cell sonication, focused ultrasound, focused ultrasound transducer, in vitro sonication, sonicator",

author = "F. Stewart and Cox, {B. F.} and G. Wang and Z. Huang and Newton, {I. P.} and Nathke, {I. S.} and M. Thanou and S. Cochran",

note = "No funding; 2016 IEEE International Ultrasonics Symposium, IUS 2016 ; Conference date: 18-09-2016 Through 21-09-2016",

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doi = "10.1109/ULTSYM.2016.7728701",

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Stewart, F, Cox, BF, Wang, G, Huang, Z, Newton, IP, Nathke, IS, Thanou, M & Cochran, S 2016, An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery. in 2016 IEEE International Ultrasonics Symposium IUS (2016). vol. 2016-November, 7728701, IEEE Computer Society, pp. 1-4, 2016 IEEE International Ultrasonics Symposium, IUS 2016, Tours, France, 18/09/16. https://doi.org/10.1109/ULTSYM.2016.7728701

TY - GEN

T1 - An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery

AU - Stewart, F.

AU - Cox, B. F.

AU - Wang, G.

AU - Huang, Z.

AU - Newton, I. P.

AU - Nathke, I. S.

AU - Thanou, M.

AU - Cochran, S.

N1 - No funding

PY - 2016/11/3

Y1 - 2016/11/3

N2 - Ultrasound is a well-established imaging modality used in clinics worldwide for many different applications. It has also experienced recent growth in interest for therapeutic applications in the form of high-intensity focused ultrasound (HIFU) or focused ultrasound (focused-US), including in capsules for use in drug delivery in the gut. The work described here aimed to support the investigation of its usefulness for drug delivery further. An in vitro sonication system was designed, comprising a focused-US transducer, a cell plate on a vertical stage and transepithelial electrical resistance (TER) meter. Miniature focused-US transducers were fabricated with PZ26 and PZ54 materials (Meggitt Sensing Systems, Kvistgaard, Denmark). The active elements in both transducers have outer diameter, OD = 5 mm, and radius of curvature, RC = 15 mm. US field mapping indicated that output acoustic pressures are in the range 25 kPa <Pac <210 kPa and acoustic powers in the range 8 mW <Powerac <80 mW. Feasibility testing with Caco-2 colon cancer cells involved measuring TER during and after sonication. Sonication produced a drop in TER of 6.7% after 6 min. Recovery to the starting TER value was observed 14 min after sonication indicating that the sonication system can be used to measure the effect of ultrasound on tissue barrier function as expected.

AB - Ultrasound is a well-established imaging modality used in clinics worldwide for many different applications. It has also experienced recent growth in interest for therapeutic applications in the form of high-intensity focused ultrasound (HIFU) or focused ultrasound (focused-US), including in capsules for use in drug delivery in the gut. The work described here aimed to support the investigation of its usefulness for drug delivery further. An in vitro sonication system was designed, comprising a focused-US transducer, a cell plate on a vertical stage and transepithelial electrical resistance (TER) meter. Miniature focused-US transducers were fabricated with PZ26 and PZ54 materials (Meggitt Sensing Systems, Kvistgaard, Denmark). The active elements in both transducers have outer diameter, OD = 5 mm, and radius of curvature, RC = 15 mm. US field mapping indicated that output acoustic pressures are in the range 25 kPa <Pac <210 kPa and acoustic powers in the range 8 mW <Powerac <80 mW. Feasibility testing with Caco-2 colon cancer cells involved measuring TER during and after sonication. Sonication produced a drop in TER of 6.7% after 6 min. Recovery to the starting TER value was observed 14 min after sonication indicating that the sonication system can be used to measure the effect of ultrasound on tissue barrier function as expected.

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M3 - Conference contribution

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VL - 2016-November

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EP - 4

BT - 2016 IEEE International Ultrasonics Symposium IUS (2016)

PB - IEEE Computer Society

T2 - 2016 IEEE International Ultrasonics Symposium, IUS 2016

Y2 - 18 September 2016 through 21 September 2016

ER -

An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery

Abstract

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Sonopill: Minimally Invasive Gastrointestinal Diagnosis and Therapy (Joint with University of Glasgow & Heriot Watt University)

Cite this

An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery

Abstract

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

Sonopill: Minimally Invasive Gastrointestinal Diagnosis and Therapy (Joint with University of Glasgow & Heriot Watt University)

Cite this