An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery

F. Stewart, B. F. Cox, G. Wang, Z. Huang, I. P. Newton, I. S. Nathke, M. Thanou, S. Cochran

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Ultrasound is a well-established imaging modality used in clinics worldwide for many different applications. It has also experienced recent growth in interest for therapeutic applications in the form of high-intensity focused ultrasound (HIFU) or focused ultrasound (focused-US), including in capsules for use in drug delivery in the gut. The work described here aimed to support the investigation of its usefulness for drug delivery further. An in vitro sonication system was designed, comprising a focused-US transducer, a cell plate on a vertical stage and transepithelial electrical resistance (TER) meter. Miniature focused-US transducers were fabricated with PZ26 and PZ54 materials (Meggitt Sensing Systems, Kvistgaard, Denmark). The active elements in both transducers have outer diameter, OD = 5 mm, and radius of curvature, RC = 15 mm. US field mapping indicated that output acoustic pressures are in the range 25 kPa <Pac <210 kPa and acoustic powers in the range 8 mW <Powerac <80 mW. Feasibility testing with Caco-2 colon cancer cells involved measuring TER during and after sonication. Sonication produced a drop in TER of 6.7% after 6 min. Recovery to the starting TER value was observed 14 min after sonication indicating that the sonication system can be used to measure the effect of ultrasound on tissue barrier function as expected.

Original languageEnglish
Title of host publication2016 IEEE International Ultrasonics Symposium IUS (2016)
PublisherIEEE Computer Society
Pages1-4
Number of pages4
Volume2016-November
ISBN (Electronic)9781467398978
DOIs
Publication statusPublished - 3 Nov 2016
Event2016 IEEE International Ultrasonics Symposium, IUS 2016 - Tours, France
Duration: 18 Sep 201621 Sep 2016

Conference

Conference2016 IEEE International Ultrasonics Symposium, IUS 2016
CountryFrance
CityTours
Period18/09/1621/09/16

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Keywords

  • cell sonication
  • focused ultrasound
  • focused ultrasound transducer
  • in vitro sonication
  • sonicator

Cite this

Stewart, F., Cox, B. F., Wang, G., Huang, Z., Newton, I. P., Nathke, I. S., Thanou, M., & Cochran, S. (2016). An in vitro sonication system for applications in ultrasound-mediated targeted drug delivery. In 2016 IEEE International Ultrasonics Symposium IUS (2016) (Vol. 2016-November, pp. 1-4). [7728701] IEEE Computer Society. https://doi.org/10.1109/ULTSYM.2016.7728701