An in vivo analysis of the localisation and interactions of human p66 DNA polymerase δ subunit

J Richard G. Pohler; Marit Otterlei; Emma Warbrick

doi:10.1186/1471-2199-6-17

An in vivo analysis of the localisation and interactions of human p66 DNA polymerase δ subunit

J Richard G. Pohler, Marit Otterlei, Emma Warbrick

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23 Citations (Scopus)

Abstract

DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely mediated through the p66 polymerase subunit. We have analysed the interactions of the human p66 DNA polymerase d subunit with PCNA and with components of the DNA polymerase d complex in vivo.

Original language	English
Pages (from-to)	17-30
Number of pages	14
Journal	BMC Molecular Biology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1186/1471-2199-6-17
Publication status	Published - 2005

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abstract = "DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely mediated through the p66 polymerase subunit. We have analysed the interactions of the human p66 DNA polymerase d subunit with PCNA and with components of the DNA polymerase d complex in vivo.",

author = "Pohler, {J Richard G.} and Marit Otterlei and Emma Warbrick",

note = " dc.publisher: BioMed Central Senior author of a paper describing mapping of the interaction domains of the p66 subunit of DNA polymerase delta with its p50co-subunit and with PCNA. Biochemical techniques and FRET technology to demonstrate and map the interactions in human cells demonstrated that these are essential functional interactions. ",

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AB - DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely mediated through the p66 polymerase subunit. We have analysed the interactions of the human p66 DNA polymerase d subunit with PCNA and with components of the DNA polymerase d complex in vivo.

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An in vivo analysis of the localisation and interactions of human p66 DNA polymerase δ subunit

Abstract

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