An in vivo analysis of the localisation and interactions of human p66 DNA polymerase δ subunit

J Richard G. Pohler; Marit Otterlei; Emma Warbrick

doi:10.1186/1471-2199-6-17

An in vivo analysis of the localisation and interactions of human p66 DNA polymerase δ subunit

J Richard G. Pohler, Marit Otterlei, Emma Warbrick

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Abstract

DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely mediated through the p66 polymerase subunit. We have analysed the interactions of the human p66 DNA polymerase d subunit with PCNA and with components of the DNA polymerase d complex in vivo.

Original language	English
Pages (from-to)	17-30
Number of pages	14
Journal	BMC Molecular Biology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1186/1471-2199-6-17
Publication status	Published - 2005

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Pohler, J. R. G., Otterlei, M., & Warbrick, E. (2005). An in vivo analysis of the localisation and interactions of human p66 DNA polymerase δ subunit. BMC Molecular Biology, 6(1), 17-30. https://doi.org/10.1186/1471-2199-6-17

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abstract = "DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely mediated through the p66 polymerase subunit. We have analysed the interactions of the human p66 DNA polymerase d subunit with PCNA and with components of the DNA polymerase d complex in vivo.",

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AB - DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely mediated through the p66 polymerase subunit. We have analysed the interactions of the human p66 DNA polymerase d subunit with PCNA and with components of the DNA polymerase d complex in vivo.

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Access to Document

10.1186/1471-2199-6-17