An in vivo analysis of the localisation and interactions of human p66 DNA polymerase δ subunit

TY - JOUR

T1 - An in vivo analysis of the localisation and interactions of human p66 DNA polymerase δ subunit

AU - Pohler, J Richard G.

AU - Otterlei, Marit

AU - Warbrick, Emma

N1 - dc.publisher: BioMed Central Senior author of a paper describing mapping of the interaction domains of the p66 subunit of DNA polymerase delta with its p50co-subunit and with PCNA. Biochemical techniques and FRET technology to demonstrate and map the interactions in human cells demonstrated that these are essential functional interactions.

PY - 2005

Y1 - 2005

N2 - DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely mediated through the p66 polymerase subunit. We have analysed the interactions of the human p66 DNA polymerase d subunit with PCNA and with components of the DNA polymerase d complex in vivo.

AB - DNA polymerase d is essential for eukaryotic DNA replication and also plays a role in DNA repair. The processivity of this polymerase complex is dependent upon its interaction with the sliding clamp PCNA and the polymerase-PCNA interaction is largely mediated through the p66 polymerase subunit. We have analysed the interactions of the human p66 DNA polymerase d subunit with PCNA and with components of the DNA polymerase d complex in vivo.

U2 - 10.1186/1471-2199-6-17

DO - 10.1186/1471-2199-6-17

M3 - Article

C2 - 16000169

VL - 6

SP - 17

EP - 30

JO - BMC Molecular Biology

JF - BMC Molecular Biology

IS - 1

ER -