An inducible chaperone adapts proteasome assembly to stress

Ariane Hanssum, Zhen Zhong, Adrien Rousseau, Agnieszka Krzyzosiak, Anna Sigurdardottir, Anne Bertolotti (Lead / Corresponding author)

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Abstract

The proteasome is essential for the selective degradation of most cellular proteins. To survive overwhelming demands on the proteasome arising during environmental stresses, cells increase proteasome abundance. Proteasome assembly is known to be complex. How stressed cells overcome this vital challenge is unknown. In an unbiased suppressor screen aimed at rescuing the defects of a yeast Rpt6 thermosensitive proteasome mutant, we identified a protein, hereafter named Adc17, as it functions as an ATPase dedicated chaperone. Adc17 interacts with the amino terminus of Rpt6 to assist formation of the Rpt6-Rpt3 ATPase pair, an early step in proteasome assembly. Adc17 is important for cell fitness, and its absence aggravates proteasome defects. The abundance of Adc17 increases upon proteasome stresses, and its function is crucial to maintain homeostatic proteasome levels. Thus, cells have mechanisms to adjust proteasome assembly when demands increase, and Adc17 is a critical effector of this process.

Original languageEnglish
Pages (from-to)566-577
Number of pages12
JournalMolecular Cell
Volume55
Issue number4
DOIs
Publication statusPublished - 21 Aug 2014

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Proteasome Endopeptidase Complex
Adenosine Triphosphatases
Proteins
Yeasts

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Hanssum, A., Zhong, Z., Rousseau, A., Krzyzosiak, A., Sigurdardottir, A., & Bertolotti, A. (2014). An inducible chaperone adapts proteasome assembly to stress. Molecular Cell, 55(4), 566-577. https://doi.org/10.1016/j.molcel.2014.06.017
Hanssum, Ariane ; Zhong, Zhen ; Rousseau, Adrien ; Krzyzosiak, Agnieszka ; Sigurdardottir, Anna ; Bertolotti, Anne. / An inducible chaperone adapts proteasome assembly to stress. In: Molecular Cell. 2014 ; Vol. 55, No. 4. pp. 566-577.
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Hanssum, A, Zhong, Z, Rousseau, A, Krzyzosiak, A, Sigurdardottir, A & Bertolotti, A 2014, 'An inducible chaperone adapts proteasome assembly to stress', Molecular Cell, vol. 55, no. 4, pp. 566-577. https://doi.org/10.1016/j.molcel.2014.06.017

An inducible chaperone adapts proteasome assembly to stress. / Hanssum, Ariane; Zhong, Zhen; Rousseau, Adrien; Krzyzosiak, Agnieszka; Sigurdardottir, Anna; Bertolotti, Anne (Lead / Corresponding author).

In: Molecular Cell, Vol. 55, No. 4, 21.08.2014, p. 566-577.

Research output: Contribution to journalArticle

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Hanssum A, Zhong Z, Rousseau A, Krzyzosiak A, Sigurdardottir A, Bertolotti A. An inducible chaperone adapts proteasome assembly to stress. Molecular Cell. 2014 Aug 21;55(4):566-577. https://doi.org/10.1016/j.molcel.2014.06.017