An intranucleolar body associated with rDNA

Saskia Hutten, Alan Prescott, John James, Stefanie Riesenberg, Severine Boulon, Yun Wah Lam, Angus I. Lamond

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    The nucleolus is the subnuclear organelle responsible for ribosome subunit biogenesis and can also act as a stress sensor. It forms around clusters of ribosomal DNA (rDNA) and is mainly organised in three subcompartments, i.e. fibrillar centre, dense fibrillar component and granular component. Here, we describe the localisation of 21 protein factors to an intranucleolar region different to these main subcompartments, called the intranucleolar body (INB). These factors include proteins involved in DNA maintenance, protein turnover, RNA metabolism, chromatin organisation and the post-translational modifiers SUMO1 and SUMO2/3. Increase in the size and number of INBs is promoted by specific types of DNA damage and depends on the functional integrity of the nucleolus. INBs are abundant in nucleoli of unstressed cells during S phase and localise in close proximity to rDNA with heterochromatic features. The data suggest the INB is linked with regulation of rDNA transcription and/or maintenance of rDNA.

    Original languageEnglish
    Pages (from-to)481-499
    Number of pages19
    JournalChromosoma
    Volume120
    Issue number5
    DOIs
    Publication statusPublished - Oct 2011

    Keywords

    • RIBOSOMAL-RNA GENES
    • POLYMERASE-I TRANSCRIPTION
    • REG-GAMMA PROTEASOME
    • DOUBLE-STRAND BREAKS
    • U6 SPLICEOSOMAL RNA
    • DNA-DAMAGE RESPONSE
    • COILED BODIES
    • PROTEIN PHOSPHATASE
    • FLUORESCENT PROTEIN
    • REPLICATION FORK

    Cite this

    Hutten, S., Prescott, A., James, J., Riesenberg, S., Boulon, S., Lam, Y. W., & Lamond, A. I. (2011). An intranucleolar body associated with rDNA. Chromosoma, 120(5), 481-499. https://doi.org/10.1007/s00412-011-0327-8
    Hutten, Saskia ; Prescott, Alan ; James, John ; Riesenberg, Stefanie ; Boulon, Severine ; Lam, Yun Wah ; Lamond, Angus I. / An intranucleolar body associated with rDNA. In: Chromosoma. 2011 ; Vol. 120, No. 5. pp. 481-499.
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    Hutten, S, Prescott, A, James, J, Riesenberg, S, Boulon, S, Lam, YW & Lamond, AI 2011, 'An intranucleolar body associated with rDNA', Chromosoma, vol. 120, no. 5, pp. 481-499. https://doi.org/10.1007/s00412-011-0327-8

    An intranucleolar body associated with rDNA. / Hutten, Saskia; Prescott, Alan; James, John; Riesenberg, Stefanie; Boulon, Severine; Lam, Yun Wah; Lamond, Angus I.

    In: Chromosoma, Vol. 120, No. 5, 10.2011, p. 481-499.

    Research output: Contribution to journalArticle

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    AU - Hutten, Saskia

    AU - Prescott, Alan

    AU - James, John

    AU - Riesenberg, Stefanie

    AU - Boulon, Severine

    AU - Lam, Yun Wah

    AU - Lamond, Angus I.

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    AB - The nucleolus is the subnuclear organelle responsible for ribosome subunit biogenesis and can also act as a stress sensor. It forms around clusters of ribosomal DNA (rDNA) and is mainly organised in three subcompartments, i.e. fibrillar centre, dense fibrillar component and granular component. Here, we describe the localisation of 21 protein factors to an intranucleolar region different to these main subcompartments, called the intranucleolar body (INB). These factors include proteins involved in DNA maintenance, protein turnover, RNA metabolism, chromatin organisation and the post-translational modifiers SUMO1 and SUMO2/3. Increase in the size and number of INBs is promoted by specific types of DNA damage and depends on the functional integrity of the nucleolus. INBs are abundant in nucleoli of unstressed cells during S phase and localise in close proximity to rDNA with heterochromatic features. The data suggest the INB is linked with regulation of rDNA transcription and/or maintenance of rDNA.

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    KW - U6 SPLICEOSOMAL RNA

    KW - DNA-DAMAGE RESPONSE

    KW - COILED BODIES

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    KW - FLUORESCENT PROTEIN

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    Hutten S, Prescott A, James J, Riesenberg S, Boulon S, Lam YW et al. An intranucleolar body associated with rDNA. Chromosoma. 2011 Oct;120(5):481-499. https://doi.org/10.1007/s00412-011-0327-8