An invasive Mimosa in India does not adopt the symbionts of its native relatives

Hukam Singh Gehlot, Nisha Tak, Muskan Kaushik, Shubhajit Mitra, Wen-Ming Chen, Nicole Poweleit, Dhereen Panwar, Neetu Poonar, Rashmita Parihar, Alkesh Tak, Indu Singh Sankhla, Archana Ojha, Satyawada Rama Rao, Marcelo F. Simon, Fabio Bueno dos Reis Junior, Natalia Perigolo, Anil K. Tripathi, Janet I. Sprent, J. Peter W. Young, Euan K. JamesPrasad Gyaneshwar

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    60 Citations (Scopus)

    Abstract

    Background and AimsThe large monophyletic genus Mimosa comprises approx. 500 species, most of which are native to the New World, with Central Brazil being the main centre of radiation. All Brazilian Mimosa spp. so far examined are nodulated by rhizobia in the betaproteobacterial genus Burkholderia. Approximately 10 Mya, transoceanic dispersal resulted in the Indian subcontinent hosting up to six endemic Mimosa spp. The nodulation ability and rhizobial symbionts of two of these, M. hamata and M. himalayana, both from north-west India, are here examined, and compared with those of M. pudica, an invasive species.MethodsNodules were collected from several locations, and examined by light and electron microscopy. Rhizobia isolated from them were characterized in terms of their abilities to nodulate the three Mimosa hosts. The molecular phylogenetic relationships of the rhizobia were determined by analysis of 16S rRNA, nifH and nodA gene sequences.Key ResultsBoth native Indian Mimosa spp. nodulated effectively in their respective rhizosphere soils. Based on 16S rRNA, nifH and nodA sequences, their symbionts were identified as belonging to the alphaproteobacterial genus Ensifer, and were closest to the 'Old World' Ensifer saheli, E. kostiensis and E. arboris. In contrast, the invasive M. pudica was predominantly nodulated by Betaproteobacteria in the genera Cupriavidus and Burkholderia. All rhizobial strains tested effectively nodulated their original hosts, but the symbionts of the native species could not nodulate M. pudica.ConclusionsThe native Mimosa spp. in India are not nodulated by the Burkholderia symbionts of their South American relatives, but by a unique group of alpha-rhizobial microsymbionts that are closely related to the 'local' Old World Ensifer symbionts of other mimosoid legumes in north-west India. They appear not to share symbionts with the invasive M. pudica, symbionts of which are mostly beta-rhizobial.
    Original languageEnglish
    Pages (from-to)179-196
    Number of pages18
    JournalAnnals of Botany
    Volume112
    Issue number1
    DOIs
    Publication statusPublished - 2013

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    Gehlot, H. S., Tak, N., Kaushik, M., Mitra, S., Chen, W-M., Poweleit, N., Panwar, D., Poonar, N., Parihar, R., Tak, A., Sankhla, I. S., Ojha, A., Rao, S. R., Simon, M. F., Reis Junior, F. B. D., Perigolo, N., Tripathi, A. K., Sprent, J. I., Young, J. P. W., ... Gyaneshwar, P. (2013). An invasive Mimosa in India does not adopt the symbionts of its native relatives. Annals of Botany, 112(1), 179-196. https://doi.org/10.1093/aob/mct112