An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies

Mihnea V. Turcanu, Sandy Cochran, Alexandru C. Moldovan, Stavros Vlatakis, Driton Vllasaliu, Maya Thanou, Inke Nathke

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    3 Citations (Scopus)


    Standard in vitro models to study intestinal drug absorption and delivery, including ultrasound (US)-mediated drug delivery, are grown from tumorous colon and rectum cells. However, the intestinal epithelium is a heterogeneous tissue and includes stem cells that differentiate into multiple cell types, including those responsible for absorption of nutrients, secretion of mucus, sensing of nutrients and immunity. Conventional cell monolayers lack this complexity. Organoid (OG)-derived cell layers may be more physiologically relevant. This paper presents a method for growing a confluent cell layer from rectal OGs on inserts and describes how this layer was used to compare the effects of US in combination with microbubbles (MBs) and US plus nanodroplets (NDs) on the intestinal barrier function and the permeation of insulin across the cell layer. Both treatments decreased the barrier function of the cell layer to about half their original values. US with MBs created a pore in the tissue and resulted in a 13-fold increase in insulin permeation compared to US with NDs.

    Original languageEnglish
    Title of host publicationIUS 2020 - International Ultrasonics Symposium, Proceedings
    Number of pages4
    ISBN (Electronic)9781728154480
    ISBN (Print)9781728154497
    Publication statusPublished - 17 Nov 2020
    Event2020 IEEE International Ultrasonics Symposium, IUS 2020 - Las Vegas, United States
    Duration: 7 Sept 202011 Sept 2020

    Publication series

    NameIEEE International Ultrasonics Symposium, IUS
    ISSN (Print)1948-5719
    ISSN (Electronic)1948-5727


    Conference2020 IEEE International Ultrasonics Symposium, IUS 2020
    Country/TerritoryUnited States
    CityLas Vegas


    • Drug delivery
    • In vitro
    • Microbubble
    • Nanodroplet
    • Organoid

    ASJC Scopus subject areas

    • Acoustics and Ultrasonics


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