An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies

Mihnea V. Turcanu; Sandy Cochran; Alexandru C. Moldovan; Stavros Vlatakis; Driton Vllasaliu; Maya Thanou; Inke Nathke

doi:10.1109/IUS46767.2020.9251401

An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies

Mihnea V. Turcanu, Sandy Cochran, Alexandru C. Moldovan, Stavros Vlatakis, Driton Vllasaliu, Maya Thanou, Inke Nathke

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

Standard in vitro models to study intestinal drug absorption and delivery, including ultrasound (US)-mediated drug delivery, are grown from tumorous colon and rectum cells. However, the intestinal epithelium is a heterogeneous tissue and includes stem cells that differentiate into multiple cell types, including those responsible for absorption of nutrients, secretion of mucus, sensing of nutrients and immunity. Conventional cell monolayers lack this complexity. Organoid (OG)-derived cell layers may be more physiologically relevant. This paper presents a method for growing a confluent cell layer from rectal OGs on inserts and describes how this layer was used to compare the effects of US in combination with microbubbles (MBs) and US plus nanodroplets (NDs) on the intestinal barrier function and the permeation of insulin across the cell layer. Both treatments decreased the barrier function of the cell layer to about half their original values. US with MBs created a pore in the tissue and resulted in a 13-fold increase in insulin permeation compared to US with NDs.

Original language	English
Title of host publication	IUS 2020 - International Ultrasonics Symposium, Proceedings
Publisher	IEEE
Number of pages	4
ISBN (Electronic)	9781728154480
ISBN (Print)	9781728154497
DOIs	https://doi.org/10.1109/IUS46767.2020.9251401
Publication status	Published - 17 Nov 2020
Event	2020 IEEE International Ultrasonics Symposium, IUS 2020 - Las Vegas, United States Duration: 7 Sept 2020 → 11 Sept 2020

Publication series

Name	IEEE International Ultrasonics Symposium, IUS
Volume	2020-September
ISSN (Print)	1948-5719
ISSN (Electronic)	1948-5727

Conference

Conference	2020 IEEE International Ultrasonics Symposium, IUS 2020
Country/Territory	United States
City	Las Vegas
Period	7/09/20 → 11/09/20

Keywords

Drug delivery
In vitro
Microbubble
Nanodroplet
Organoid

ASJC Scopus subject areas

Acoustics and Ultrasonics

Access to Document

10.1109/IUS46767.2020.9251401

Cite this

Turcanu, M. V., Cochran, S., Moldovan, A. C., Vlatakis, S., Vllasaliu, D., Thanou, M., & Nathke, I. (2020). An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies. In IUS 2020 - International Ultrasonics Symposium, Proceedings Article 9251401 (IEEE International Ultrasonics Symposium, IUS; Vol. 2020-September). IEEE. https://doi.org/10.1109/IUS46767.2020.9251401

@inproceedings{33fa6eca863040939e7dc2a49090769d,

title = "An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies",

abstract = "Standard in vitro models to study intestinal drug absorption and delivery, including ultrasound (US)-mediated drug delivery, are grown from tumorous colon and rectum cells. However, the intestinal epithelium is a heterogeneous tissue and includes stem cells that differentiate into multiple cell types, including those responsible for absorption of nutrients, secretion of mucus, sensing of nutrients and immunity. Conventional cell monolayers lack this complexity. Organoid (OG)-derived cell layers may be more physiologically relevant. This paper presents a method for growing a confluent cell layer from rectal OGs on inserts and describes how this layer was used to compare the effects of US in combination with microbubbles (MBs) and US plus nanodroplets (NDs) on the intestinal barrier function and the permeation of insulin across the cell layer. Both treatments decreased the barrier function of the cell layer to about half their original values. US with MBs created a pore in the tissue and resulted in a 13-fold increase in insulin permeation compared to US with NDs.",

keywords = "Drug delivery, In vitro, Microbubble, Nanodroplet, Organoid",

author = "Turcanu, {Mihnea V.} and Sandy Cochran and Moldovan, {Alexandru C.} and Stavros Vlatakis and Driton Vllasaliu and Maya Thanou and Inke Nathke",

note = "Funding Information: This work was supported by the UK Engineering and Physical Sciences Research Council (EPSRC) under grant number EP/K034537 “Sonopill” (www.gla.ac.uk/research/sonopill/), the UK Biotechnology and Biological Sciences Research Council (BBSRC), project reference 1810028, and Curileum Discovery Limited (Harrow, UK). Publisher Copyright: {\textcopyright} 2020 IEEE. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.; 2020 IEEE International Ultrasonics Symposium, IUS 2020 ; Conference date: 07-09-2020 Through 11-09-2020",

year = "2020",

month = nov,

day = "17",

doi = "10.1109/IUS46767.2020.9251401",

language = "English",

isbn = "9781728154497",

series = "IEEE International Ultrasonics Symposium, IUS",

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Turcanu, MV, Cochran, S, Moldovan, AC, Vlatakis, S, Vllasaliu, D, Thanou, M & Nathke, I 2020, An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies. in IUS 2020 - International Ultrasonics Symposium, Proceedings., 9251401, IEEE International Ultrasonics Symposium, IUS, vol. 2020-September, IEEE, 2020 IEEE International Ultrasonics Symposium, IUS 2020, Las Vegas, United States, 7/09/20. https://doi.org/10.1109/IUS46767.2020.9251401

An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies. / Turcanu, Mihnea V.; Cochran, Sandy; Moldovan, Alexandru C. et al.
IUS 2020 - International Ultrasonics Symposium, Proceedings. IEEE, 2020. 9251401 (IEEE International Ultrasonics Symposium, IUS; Vol. 2020-September).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies

AU - Turcanu, Mihnea V.

AU - Cochran, Sandy

AU - Moldovan, Alexandru C.

AU - Vlatakis, Stavros

AU - Vllasaliu, Driton

AU - Thanou, Maya

AU - Nathke, Inke

N1 - Funding Information: This work was supported by the UK Engineering and Physical Sciences Research Council (EPSRC) under grant number EP/K034537 “Sonopill” (www.gla.ac.uk/research/sonopill/), the UK Biotechnology and Biological Sciences Research Council (BBSRC), project reference 1810028, and Curileum Discovery Limited (Harrow, UK). Publisher Copyright: © 2020 IEEE. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11/17

Y1 - 2020/11/17

N2 - Standard in vitro models to study intestinal drug absorption and delivery, including ultrasound (US)-mediated drug delivery, are grown from tumorous colon and rectum cells. However, the intestinal epithelium is a heterogeneous tissue and includes stem cells that differentiate into multiple cell types, including those responsible for absorption of nutrients, secretion of mucus, sensing of nutrients and immunity. Conventional cell monolayers lack this complexity. Organoid (OG)-derived cell layers may be more physiologically relevant. This paper presents a method for growing a confluent cell layer from rectal OGs on inserts and describes how this layer was used to compare the effects of US in combination with microbubbles (MBs) and US plus nanodroplets (NDs) on the intestinal barrier function and the permeation of insulin across the cell layer. Both treatments decreased the barrier function of the cell layer to about half their original values. US with MBs created a pore in the tissue and resulted in a 13-fold increase in insulin permeation compared to US with NDs.

AB - Standard in vitro models to study intestinal drug absorption and delivery, including ultrasound (US)-mediated drug delivery, are grown from tumorous colon and rectum cells. However, the intestinal epithelium is a heterogeneous tissue and includes stem cells that differentiate into multiple cell types, including those responsible for absorption of nutrients, secretion of mucus, sensing of nutrients and immunity. Conventional cell monolayers lack this complexity. Organoid (OG)-derived cell layers may be more physiologically relevant. This paper presents a method for growing a confluent cell layer from rectal OGs on inserts and describes how this layer was used to compare the effects of US in combination with microbubbles (MBs) and US plus nanodroplets (NDs) on the intestinal barrier function and the permeation of insulin across the cell layer. Both treatments decreased the barrier function of the cell layer to about half their original values. US with MBs created a pore in the tissue and resulted in a 13-fold increase in insulin permeation compared to US with NDs.

KW - Drug delivery

KW - In vitro

KW - Microbubble

KW - Nanodroplet

KW - Organoid

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U2 - 10.1109/IUS46767.2020.9251401

DO - 10.1109/IUS46767.2020.9251401

M3 - Conference contribution

AN - SCOPUS:85097879719

SN - 9781728154497

T3 - IEEE International Ultrasonics Symposium, IUS

BT - IUS 2020 - International Ultrasonics Symposium, Proceedings

PB - IEEE

T2 - 2020 IEEE International Ultrasonics Symposium, IUS 2020

Y2 - 7 September 2020 through 11 September 2020

ER -

An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Sonopill: Minimally Invasive Gastrointestinal Diagnosis and Therapy (Joint with University of Glasgow & Heriot Watt University)

Cite this

An Organoid-derived Cell Layer as an in vitro Model for US-mediated Drug Delivery Studies

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

Sonopill: Minimally Invasive Gastrointestinal Diagnosis and Therapy (Joint with University of Glasgow & Heriot Watt University)

Cite this