Analysis of Barotactic and Chemotactic Guidance Cues on Directional Decision-making of Dictyostelium Cells in Confined Environments

Yuri Belotti, David McGloin, Kees Weijer (Lead / Corresponding author)

Research output: Contribution to journalArticle

Abstract

Neutrophils and dendritic cells when migrating in confined environments have been shown to actuate a directional choice toward paths of least hydraulic resistance (barotaxis), in some cases overriding chemotactic responses. Here, we investigate whether this barotactic response is conserved in the more primitive model organism Dictyostelium discoideum using a microfluidic chip design. This design allowed us to monitor the behavior of single cells via live imaging when confronted with bifurcating microchannels, presenting different combinations of hydraulic and chemical stimuli. Under the conditions employed we find no evidence in support of a barotactic response; the cells base their directional choices on the chemotactic cues. When the cells are confronted by a microchannel bifurcation, they often split their leading edge and start moving into both channels, before a decision is made to move into one and retract from the other channel. Analysis of this decision-making process has shown that cells in steeper nonhydrolyzable adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer (cAMPS) gradients move faster and split more readily. Furthermore, there exists a highly significant strong correlation between the velocity of the pseudopod moving up the cAMPS gradient to the total velocity of the pseudopods moving up and down the gradient over a large range of velocities. This suggests a role for a critical cortical tension gradient in the directional decision-making process.
Original languageEnglish
Pages (from-to)25553-25559
Number of pages7
JournalProceedings of the National Academy of Sciences
Volume117
Issue number41
Early online date30 Sep 2020
DOIs
Publication statusPublished - 13 Oct 2020

Keywords

  • chemotaxis
  • barotaxis
  • microfluidics
  • cell migration

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