Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats

J. Provan; A. Kumar; L. Shepherd; W. Powell; R. Waugh

doi:10.1007/BF01890866

Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats

J. Provan, A. Kumar, L. Shepherd, W. Powell, R. Waugh

Plant Sciences

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high- resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids.

Original language	English
Pages (from-to)	196-199
Number of pages	4
Journal	Plant Cell Reports
Volume	16
Issue number	3-4
DOIs	https://doi.org/10.1007/BF01890866
Publication status	Published - Dec 1996

Keywords

potato
simple sequence repeats
somatic hybrids

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

Access to Document

10.1007/BF01890866

Cite this

@article{29a8744c0dae42a6b940f936a3a99cfc,

title = "Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats",

abstract = "We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high- resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids.",

keywords = "potato, simple sequence repeats, somatic hybrids",

author = "J. Provan and A. Kumar and L. Shepherd and W. Powell and R. Waugh",

year = "1996",

month = dec,

doi = "10.1007/BF01890866",

language = "English",

volume = "16",

pages = "196--199",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Verlag",

number = "3-4",

}

TY - JOUR

T1 - Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats

AU - Provan, J.

AU - Kumar, A.

AU - Shepherd, L.

AU - Powell, W.

AU - Waugh, R.

PY - 1996/12

Y1 - 1996/12

N2 - We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high- resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids.

AB - We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high- resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids.

KW - potato

KW - simple sequence repeats

KW - somatic hybrids

UR - http://www.scopus.com/inward/record.url?scp=0030472385&partnerID=8YFLogxK

U2 - 10.1007/BF01890866

DO - 10.1007/BF01890866

M3 - Article

AN - SCOPUS:0030472385

SN - 0721-7714

VL - 16

SP - 196

EP - 199

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 3-4

ER -

Analysis of intra-specific somatic hybrids of potato (Solanum tuberosum) using simple sequence repeats

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this