Analysis of microbial diversity in oligotrophic microbial fuel cells using 16S rDNA sequences

Nguyet Thu Phung, Jiyoung Lee, Kui Hyun Kang, In Seop Chang, Geoffrey Michael Gadd, Byung Hong Kim (Lead / Corresponding author)

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148 Citations (Scopus)

Abstract

Molecular ecological techniques were applied to analyze the bacterial diversity of two oligotrophic microbial fuel cells (MFCs) enriched using river water or artificial wastewater (AWW) as fuel. Denaturing gradient gel electrophoresis (DGGE) of the PCR amplified 16S rDNA showed that different microbial communities were present in the two MFCs and these were different from the river sediment used to initiate the enrichment. Nearly complete 16S rDNA was amplified and sequenced. Over 80% of the clones were Proteobacteria. Betaproteobacteria were the dominant clones (46.2%) in MFCs fed with river water, and about 64.4% of the clones in MFCs fed with AWW were Alphaproteobacteria. Actinobacteria were found only in the MFC fed with AWW, and Deltaproteobacteria, Acidobacteria, Chloroflexi and Verrucomicrobia in the MFC fed with river water. Many clones were related to uncultured bacteria, some with homology less than 95%, indicating that many novel bacteria were enriched in the oligotrophic MFCs.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalFEMS Microbiology Letters
Volume233
Issue number1
Early online date10 Feb 2004
DOIs
Publication statusPublished - 1 Apr 2004

Keywords

  • 16S rDNA
  • DGGE
  • Microbial diversity
  • Microbial fuel cell
  • Oligotrophic

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