@inbook{bb033107e7a64776bb6593847a70cd92,
title = "Analysis of small GTPase function in trypanosomes",
abstract = "Trypanosomatids are protozoan parasites, of interest due to both their disease burden and deeply divergent position within the eukaryotic lineage. The African trypanosome, Trypanosoma brucei, has emerged as a very amenable model system, with a considerable toolbox of methods available, including inducible overexpression, RNA interference, and a completed genome. Here we describe some of the special considerations that need to be addressed when studying trypanosome gene function, and in particular small GTPases; we provide protocols for transfection, RNA interference, overexpression and basic transport assays, in addition to an overview of available vectors, cell lines, and strategies.",
keywords = "GENOME, VARIANT SURFACE GLYCOPROTEIN, TRAFFICKING, PROTEIN, BLOOD-STREAM FORMS, AFRICAN TRYPANOSOME, SYSTEM, EXPRESSION, RNA INTERFERENCE, BRUCEI",
author = "Field, {Mark C.} and David Horn and Mark Carrington",
year = "2008",
doi = "10.1016/S0076-6879(07)38005-1",
language = "English",
isbn = "9780123739681",
series = "Methods in Enzymology",
publisher = "Academic Press",
pages = "57--76",
editor = "W. Balch and Der, {Channing J.} and Alan Hall",
booktitle = "Small GTPases in disease. Part A",
}