Analysis of small GTPase function in trypanosomes

Mark C. Field, David Horn, Mark Carrington

    Research output: Chapter in Book/Report/Conference proceedingChapter

    4 Citations (Scopus)

    Abstract

    Trypanosomatids are protozoan parasites, of interest due to both their disease burden and deeply divergent position within the eukaryotic lineage. The African trypanosome, Trypanosoma brucei, has emerged as a very amenable model system, with a considerable toolbox of methods available, including inducible overexpression, RNA interference, and a completed genome. Here we describe some of the special considerations that need to be addressed when studying trypanosome gene function, and in particular small GTPases; we provide protocols for transfection, RNA interference, overexpression and basic transport assays, in addition to an overview of available vectors, cell lines, and strategies.

    Original languageEnglish
    Title of host publicationSmall GTPases in disease. Part A
    EditorsW. Balch, Channing J. Der, Alan Hall
    Place of PublicationAmsterdam
    PublisherAcademic Press
    Pages57-76
    Number of pages20
    ISBN (Print)9780123739681
    DOIs
    Publication statusPublished - 2008

    Publication series

    NameMethods in Enzymology
    PublisherAcademic Press
    Volume438
    ISSN (Print)0076-6879

    Keywords

    • GENOME
    • VARIANT SURFACE GLYCOPROTEIN
    • TRAFFICKING
    • PROTEIN
    • BLOOD-STREAM FORMS
    • AFRICAN TRYPANOSOME
    • SYSTEM
    • EXPRESSION
    • RNA INTERFERENCE
    • BRUCEI

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