Analysis of the CD33-related siglec family reveals that Siglec-9 is an endocytic receptor expressed on subsets of acute myeloid leukemia cells and absent from normal hematopoietic progenitors

Bjoern Biedermann, Diana Gil, David T. Bowen, Paul R. Crocker

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    36 Citations (Scopus)

    Abstract

    CD33 (Siglec-3) is expressed on most acute myeloid leukemia (AML) cells and is currently being exploited as a therapeutic target. The purpose of this study was to investigate the expression pattern and potential utility of the seven recently described CD33-related siglecs as markers in AML. Besides CD33, Siglec-9 was the most highly expressed, particularly on AML cells with features of monocytic differentiation that also expressed Siglecs-5 and -7. Siglec-9 was absent from normal bone marrow myeloid progenitors but present on monocytic precursors. Using primary AML cells or transfected rat basophilic leukemia cells, Siglec-9 mediated rapid endocytosis of anti-Siglec-9 mAb. In contrast to CD33 and Siglec-5, levels of soluble Siglec-9 were low or undetectable in bone marrow plasma from AML patients and serum from normal donors. These features suggest that Siglec-9 provides not only a useful marker for certain subsets of AML, but also a potential therapeutic target.
    Original languageEnglish
    Pages (from-to)211-20
    Number of pages10
    JournalLeukemia Research
    Volume31
    Issue number2
    DOIs
    Publication statusPublished - Feb 2007

    Keywords

    • Acute Disease
    • Antigens, CD
    • Antigens, Differentiation, Myelomonocytic
    • Flow Cytometry
    • Hematopoietic Stem Cells
    • Humans
    • Lectins
    • Leukemia, Myeloid
    • Sialic Acid Binding Ig-like Lectin 3
    • Sialic Acid Binding Immunoglobulin-like Lectins
    • Tumor Markers, Biological

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