Analysis of the genomic l rna segment from lymphocytic choriomeningitis virus

Mandaleshwar K. Singh, Frances V. Fuller-Pace, Michael J. Buchmeier, Peter J. Southern

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The arenavirus genomic L RNA segment represents approximately 70% of the viral genetic material but current understanding of the organization, regulation, and functioning of the viral L products remains limited. Biological studies with reassortant viruses have implicated the L RNA segment in the lethal infection of adult guinea pigs produced by LCMV-WE but no further explanation of the pathogenic process is presently available. We have initiated a detailed molecular analysis of LCMV L products based on construction and characterization of L-specific cDNA clones and synthesis of L-specific hybridization probes. Nucleotide sequencing studies have allowed the derivation of a partial amino acid sequence for a predicted L protein and antisera raised against synthetic peptides have demonstrated an L protein in Western blotting experiments. Using this approach we have identified a single high molecular weight protein (approximately 200,000 Da) in purified virions and in viral ribonucleoprotein complexes extracted from acutely infected tissue culture cells. This L protein is translated from a nonpolyadenylated, genomic complementary L mRNA and potentially represents part or all of the viral RNA-dependent RNA polymerase.

Original languageEnglish
Pages (from-to)448-456
Number of pages9
JournalVirology
Volume161
Issue number2
DOIs
Publication statusPublished - 1 Dec 1987

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Lymphocytic choriomeningitis virus
RNA
Proteins
Reassortant Viruses
Arenavirus
RNA Replicase
Ribonucleoproteins
Viral RNA
Virion
Immune Sera
Amino Acid Sequence
Guinea Pigs
Nucleotides
Complementary DNA
Clone Cells
Cell Culture Techniques
Molecular Weight
Western Blotting
Messenger RNA
Peptides

Cite this

Singh, Mandaleshwar K. ; Fuller-Pace, Frances V. ; Buchmeier, Michael J. ; Southern, Peter J. / Analysis of the genomic l rna segment from lymphocytic choriomeningitis virus. In: Virology. 1987 ; Vol. 161, No. 2. pp. 448-456.
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Analysis of the genomic l rna segment from lymphocytic choriomeningitis virus. / Singh, Mandaleshwar K.; Fuller-Pace, Frances V.; Buchmeier, Michael J.; Southern, Peter J.

In: Virology, Vol. 161, No. 2, 01.12.1987, p. 448-456.

Research output: Contribution to journalArticle

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