Analysis of the impact of sex and age on the variation in the prevalence of antinuclear autoantibodies in Polish population: a nationwide observational, cross-sectional study

Paweł Krzemień (Lead / Corresponding author), Sławomir Kasperczyk, Maciej Banach, Aleksandra Kasperczyk, Michał Dobrakowski, Tomasz Tomasik, Adam Windak, Mirosław Mastej, Alberico Catapano, Kausik K. Ray, Dimitri P. Mikhailidis, Peter P. Toth, George Howard, Gregory Y. H. Lip, Maciej Tomaszewski, Fadi J. Charchar, Naveed Sattar, Bryan Williams, Thomas M. MacDonald, Peter E. PensonJacek J. Jóźwiak,

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    Abstract

    The detection of antinuclear autoantibody (ANA) is dependent on many factors and varies between the populations. The aim of the study was first to assess the prevalence of ANA in the Polish adult population depending on age, sex and the cutoff threshold used for the results obtained. Second, we estimated the occurrence of individual types of ANA-staining patterns. We tested 1731 patient samples using commercially available IIFA using two cutoff thresholds of 1:100 and 1:160. We found ANA in 260 participants (15.0%), but the percentage of positive results strongly depended on the cutoff level. For a cutoff threshold 1:100, the positive population was 19.5% and for the 1:160 cutoff threshold, it was 11.7%. The most prevalent ANA-staining pattern was AC-2 Dense Fine speckled (50%), followed by AC-21 Reticular/AMA (14.38%) ANA more common in women (72%); 64% of ANA-positive patients were over 50 years of age. ANA prevalence in the Polish population is at a level observed in other highly developed countries and is more prevalent in women and elderly individuals. To reduce the number of positive results released, we suggest that Polish laboratories should set 1:160 as the cutoff threshold.

    Original languageEnglish
    Pages (from-to)261-271
    Number of pages11
    JournalRheumatology international
    Volume42
    Early online date10 Nov 2021
    DOIs
    Publication statusPublished - Feb 2022

    Keywords

    • Age
    • Antinuclear antibodies
    • Cutoff threshold
    • Indirect immunofluorescence assay
    • Prevalence
    • Sex

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Rheumatology
    • Immunology

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