Antagonism of 5-HT3 receptor mediated currents in murine N1E-115 neuroblastoma cells by (+)-tubocurarine

John A. Peters, Hilary M. Malone, Jeremy J. Lambert (Lead / Corresponding author)

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36 Citations (Scopus)

Abstract

5-HT3 receptor-mediated membrane currents were recorded from voltage-clamped clonal N1E-115 neuroblastoma cells. The inward current response to ionophoretically applied serotonin (5-HT) was reversibly antagonised by the 5-HT3 receptor antagonists GR 38032F and metoclopramide with IC50 values of 0.2 nM and 14 nM, respectively. Low concentrations of (+)-tubocurarine ((+)-Tc) also blocked the response to 5-HT (IC50 = 0.8 nM), but other nicotinic cholinoceptor antagonists (gallamine, vecuronium and trimetaphan) were ineffective when applied at a relatively high concentration (1 μM). Blockade by (+)-Tc was neither voltage- nor use-dependent, suggesting that (+)-Tc does not block 5-HT-activated ion channels in N1E-115 cells.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalNeuroscience Letters
Volume110
Issue number1-2
DOIs
Publication statusPublished - 2 Mar 1990

Keywords

  • (+)-Tubocurarine
  • GR 38032F
  • Metoclopramide
  • N1E-115 neuroblastoma cell
  • Nicotinic receptor antagonist
  • Serotonin
  • Serotonin (5-HT) receptor

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