Antagonism of 5-HT3 receptor mediated currents in murine N1E-115 neuroblastoma cells by (+)-tubocurarine

John A. Peters, Hilary M. Malone, Jeremy J. Lambert (Lead / Corresponding author)

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

5-HT3 receptor-mediated membrane currents were recorded from voltage-clamped clonal N1E-115 neuroblastoma cells. The inward current response to ionophoretically applied serotonin (5-HT) was reversibly antagonised by the 5-HT3 receptor antagonists GR 38032F and metoclopramide with IC50 values of 0.2 nM and 14 nM, respectively. Low concentrations of (+)-tubocurarine ((+)-Tc) also blocked the response to 5-HT (IC50 = 0.8 nM), but other nicotinic cholinoceptor antagonists (gallamine, vecuronium and trimetaphan) were ineffective when applied at a relatively high concentration (1 μM). Blockade by (+)-Tc was neither voltage- nor use-dependent, suggesting that (+)-Tc does not block 5-HT-activated ion channels in N1E-115 cells.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalNeuroscience Letters
Volume110
Issue number1-2
DOIs
Publication statusPublished - 2 Mar 1990

Fingerprint

Receptors, Serotonin, 5-HT3
Tubocurarine
Neuroblastoma
Serotonin
Inhibitory Concentration 50
Trimethaphan
Gallamine Triethiodide
Nicotinic Antagonists
Serotonin 5-HT3 Receptor Antagonists
Vecuronium Bromide
Ondansetron
Metoclopramide
Cholinergic Receptors
Ion Channels
Membranes

Keywords

  • (+)-Tubocurarine
  • GR 38032F
  • Metoclopramide
  • N1E-115 neuroblastoma cell
  • Nicotinic receptor antagonist
  • Serotonin
  • Serotonin (5-HT) receptor

Cite this

@article{67237b2c910c41c1ae9a628fb449d0f3,
title = "Antagonism of 5-HT3 receptor mediated currents in murine N1E-115 neuroblastoma cells by (+)-tubocurarine",
abstract = "5-HT3 receptor-mediated membrane currents were recorded from voltage-clamped clonal N1E-115 neuroblastoma cells. The inward current response to ionophoretically applied serotonin (5-HT) was reversibly antagonised by the 5-HT3 receptor antagonists GR 38032F and metoclopramide with IC50 values of 0.2 nM and 14 nM, respectively. Low concentrations of (+)-tubocurarine ((+)-Tc) also blocked the response to 5-HT (IC50 = 0.8 nM), but other nicotinic cholinoceptor antagonists (gallamine, vecuronium and trimetaphan) were ineffective when applied at a relatively high concentration (1 μM). Blockade by (+)-Tc was neither voltage- nor use-dependent, suggesting that (+)-Tc does not block 5-HT-activated ion channels in N1E-115 cells.",
keywords = "(+)-Tubocurarine, GR 38032F, Metoclopramide, N1E-115 neuroblastoma cell, Nicotinic receptor antagonist, Serotonin, Serotonin (5-HT) receptor",
author = "Peters, {John A.} and Malone, {Hilary M.} and Lambert, {Jeremy J.}",
year = "1990",
month = "3",
day = "2",
doi = "10.1016/0304-3940(90)90796-C",
language = "English",
volume = "110",
pages = "107--112",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Antagonism of 5-HT3 receptor mediated currents in murine N1E-115 neuroblastoma cells by (+)-tubocurarine

AU - Peters, John A.

AU - Malone, Hilary M.

AU - Lambert, Jeremy J.

PY - 1990/3/2

Y1 - 1990/3/2

N2 - 5-HT3 receptor-mediated membrane currents were recorded from voltage-clamped clonal N1E-115 neuroblastoma cells. The inward current response to ionophoretically applied serotonin (5-HT) was reversibly antagonised by the 5-HT3 receptor antagonists GR 38032F and metoclopramide with IC50 values of 0.2 nM and 14 nM, respectively. Low concentrations of (+)-tubocurarine ((+)-Tc) also blocked the response to 5-HT (IC50 = 0.8 nM), but other nicotinic cholinoceptor antagonists (gallamine, vecuronium and trimetaphan) were ineffective when applied at a relatively high concentration (1 μM). Blockade by (+)-Tc was neither voltage- nor use-dependent, suggesting that (+)-Tc does not block 5-HT-activated ion channels in N1E-115 cells.

AB - 5-HT3 receptor-mediated membrane currents were recorded from voltage-clamped clonal N1E-115 neuroblastoma cells. The inward current response to ionophoretically applied serotonin (5-HT) was reversibly antagonised by the 5-HT3 receptor antagonists GR 38032F and metoclopramide with IC50 values of 0.2 nM and 14 nM, respectively. Low concentrations of (+)-tubocurarine ((+)-Tc) also blocked the response to 5-HT (IC50 = 0.8 nM), but other nicotinic cholinoceptor antagonists (gallamine, vecuronium and trimetaphan) were ineffective when applied at a relatively high concentration (1 μM). Blockade by (+)-Tc was neither voltage- nor use-dependent, suggesting that (+)-Tc does not block 5-HT-activated ion channels in N1E-115 cells.

KW - (+)-Tubocurarine

KW - GR 38032F

KW - Metoclopramide

KW - N1E-115 neuroblastoma cell

KW - Nicotinic receptor antagonist

KW - Serotonin

KW - Serotonin (5-HT) receptor

UR - http://www.scopus.com/inward/record.url?scp=0025193637&partnerID=8YFLogxK

U2 - 10.1016/0304-3940(90)90796-C

DO - 10.1016/0304-3940(90)90796-C

M3 - Article

VL - 110

SP - 107

EP - 112

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-2

ER -