Antibacterial proteins in rainbow trout: Oncorhynchus mykiss

Valerie J. Smith, Jorge M. O. Fernandes, Sarah J. Jones, Graham D. Kemp, Mary F. Tatner

    Research output: Contribution to journalArticle

    82 Citations (Scopus)

    Abstract

    Antibacterial proteins are an important part of the innate immune system for all animals. They have been extensively studied in mammals, amphibians and invertebrates, but have received only scant attention in fish. Their expression and processing, however, provide a way of monitoring defence vigour during development or with seasonal changes in physiology. The aim of the present work was to identify and characterise antibacterial proteins in rainbow trout. In vitro analyses of extracts of the peripheral blood leucocytes, head kidney leucocytes and mucus from adult unstimulated (non-immune) fish showed marked antibacterial activity against Gram positive bacteria. Fractionation by ion exchange chromatography and RP-HPLC of head kidney extracts showed the presence of two forms of lysozyme but no constitutively expressed antimicrobial proteins of <10 kDa. By contrast, chromatographic analyses of mucus revealed at least four antibacterial proteins. Two are conventional lysozymes, a third is an unusual lysozyme-like protein with a low isoelectric point, and the fourth is a highly hydrophobic, cationic peptide of c. 3 kDa.
    Original languageEnglish
    Pages (from-to)243-260
    Number of pages18
    JournalFish and Shellfish Immunology
    Volume10
    Issue number3
    DOIs
    Publication statusPublished - Apr 2000

    Fingerprint

    antibacterial proteins
    lysozyme
    rainbow
    Oncorhynchus mykiss
    mucus
    Muramidase
    protein
    leukocytes
    kidneys
    antimicrobial proteins
    Proteins
    Fish
    extracts
    ion exchange chromatography
    isoelectric point
    Gram-positive bacteria
    fish
    vigor
    amphibians
    fractionation

    Cite this

    Smith, Valerie J. ; Fernandes, Jorge M. O. ; Jones, Sarah J. ; Kemp, Graham D. ; Tatner, Mary F. / Antibacterial proteins in rainbow trout : Oncorhynchus mykiss. In: Fish and Shellfish Immunology. 2000 ; Vol. 10, No. 3. pp. 243-260.
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    abstract = "Antibacterial proteins are an important part of the innate immune system for all animals. They have been extensively studied in mammals, amphibians and invertebrates, but have received only scant attention in fish. Their expression and processing, however, provide a way of monitoring defence vigour during development or with seasonal changes in physiology. The aim of the present work was to identify and characterise antibacterial proteins in rainbow trout. In vitro analyses of extracts of the peripheral blood leucocytes, head kidney leucocytes and mucus from adult unstimulated (non-immune) fish showed marked antibacterial activity against Gram positive bacteria. Fractionation by ion exchange chromatography and RP-HPLC of head kidney extracts showed the presence of two forms of lysozyme but no constitutively expressed antimicrobial proteins of <10 kDa. By contrast, chromatographic analyses of mucus revealed at least four antibacterial proteins. Two are conventional lysozymes, a third is an unusual lysozyme-like protein with a low isoelectric point, and the fourth is a highly hydrophobic, cationic peptide of c. 3 kDa.",
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    Antibacterial proteins in rainbow trout : Oncorhynchus mykiss. / Smith, Valerie J.; Fernandes, Jorge M. O.; Jones, Sarah J.; Kemp, Graham D.; Tatner, Mary F.

    In: Fish and Shellfish Immunology, Vol. 10, No. 3, 04.2000, p. 243-260.

    Research output: Contribution to journalArticle

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    AU - Tatner, Mary F.

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