Antigen processing and class II MHC peptide-loading compartments in human B-lymphoblastoid cells

Michele A. West, John M. Lucocq, Colin Watts

    Research output: Contribution to journalArticlepeer-review

    344 Citations (Scopus)

    Abstract

    The peptide/class II major histocompatibility complex (MHC) complexes recognized by CD4+ T cells have been characterized at the structural1 and biochemical2-4 levels and studies on the transport and maturation of class II MHC indicate that specialized sites may be involved in peptide acquisition5-11. Here we report the characterization of the compartments involved in antigen processing and class II MHC loading relative to distinct functional domains of the endocytic pathway in antigen-specific human B lymphocytes12-14. Peroxidase-mediated crosslinking analysis15 in intact cells demonstrates that peptide loading of class II MHC takes place in a compartment accessible to membrane immuno-globulin but not to transferrin receptors, although processing may be initiated within the latter domain. The density of membrane vesicles carrying newly assembled class II MHC complexes was distinct from early and late endosomes and dense lysosomes. Endocytosed antigen-gold complexes enter a class II MHC-rich compartment morphologically very similar to that described previously9 and within the time frame of biochemically detectable peptide loading.

    Original languageEnglish
    Pages (from-to)147-151
    Number of pages5
    JournalNature
    Volume369
    Issue number6476
    DOIs
    Publication statusPublished - 12 May 1994

    ASJC Scopus subject areas

    • General

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