TY - JOUR
T1 - Antileishmanial effects of Crotalaria spectabilis Roth aqueous extracts on Leishmania amazonensis
AU - Pacheco, Juliana S.
AU - Teixeira, Érika Maria G. F.
AU - Paschoal, Ramon G.
AU - Torres-Santos, Eduardo Caio
AU - De Simone, Salvatore Giovanni
AU - Da Silva-López, Raquel Elisa
N1 - Funding Information:
This study was supported by CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Fundação de Apoio para Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E-26/110.139/2009).
PY - 2023
Y1 - 2023
N2 - Fifteen polar extracts from leaf, seed, pod, stem, flower and root of Crotalaria spectabilis were prepared using aqueous systems, based on the principles of green chemistry, and showed different protease inhibitor (PI) activities on trypsin, papain, pepsin and the extracellular L. amazonensis serine protease (LSPIII). The most pronounced inhibitory effect on LSPIII was observed in leaf (CS-P), root, stem, flower (CS-FPVPP) and pod (CS-VA) extracts. Crotalaria extracts exhibited low cytotoxicity on macrophages; however, they decreased the viability of L. amazonensis promastigotes and amastigotes, as observed in leaf (CS-AE, CS-P, CS-T and CS-PVPP), seed (CS-ST), flower and root (CS-RA) extracts. CS-P was chosen to study PI and secondary metabolites and a 10-12 kDa protein, analyzed by mass spectrometry, was identified as a serine PI homologous with papaya latex serine PI. Glycosylated flavonoids, such as quercetins, vitexin and tricin were the major secondary metabolites of CS-P. The presence of PIs in C. spectabilis is a new finding, especially in other organs than seeds since PIs have been reported only in seed legumes. Besides, this is the first report of antileishmanial activity of C. spectabilis extracts and the identification of serine polypeptide PI and glycosylated flavonoids from leaf.
AB - Fifteen polar extracts from leaf, seed, pod, stem, flower and root of Crotalaria spectabilis were prepared using aqueous systems, based on the principles of green chemistry, and showed different protease inhibitor (PI) activities on trypsin, papain, pepsin and the extracellular L. amazonensis serine protease (LSPIII). The most pronounced inhibitory effect on LSPIII was observed in leaf (CS-P), root, stem, flower (CS-FPVPP) and pod (CS-VA) extracts. Crotalaria extracts exhibited low cytotoxicity on macrophages; however, they decreased the viability of L. amazonensis promastigotes and amastigotes, as observed in leaf (CS-AE, CS-P, CS-T and CS-PVPP), seed (CS-ST), flower and root (CS-RA) extracts. CS-P was chosen to study PI and secondary metabolites and a 10-12 kDa protein, analyzed by mass spectrometry, was identified as a serine PI homologous with papaya latex serine PI. Glycosylated flavonoids, such as quercetins, vitexin and tricin were the major secondary metabolites of CS-P. The presence of PIs in C. spectabilis is a new finding, especially in other organs than seeds since PIs have been reported only in seed legumes. Besides, this is the first report of antileishmanial activity of C. spectabilis extracts and the identification of serine polypeptide PI and glycosylated flavonoids from leaf.
KW - Crotalaria
KW - Fabaceae
KW - Serine Proteinase Inhibitors
KW - Antiprotozoal Agents
KW - Flavonoids
KW - Leishmania
KW - Serine
KW - Leishmania pro-teases
KW - aqueous extracts
KW - Crotalaria spectabilis
KW - protease inhibitors
KW - Leishmania viability
KW - flavonoids
UR - http://www.scopus.com/inward/record.url?scp=85169999078&partnerID=8YFLogxK
U2 - 10.1590/0001-3765202320220613
DO - 10.1590/0001-3765202320220613
M3 - Article
C2 - 37672397
SN - 0001-3765
VL - 95
JO - Anais da Academia Brasileira de Ciências
JF - Anais da Academia Brasileira de Ciências
IS - Supplement 1
M1 - e20220613
ER -