Antileishmanial effects of Crotalaria spectabilis Roth aqueous extracts on Leishmania amazonensis

TY - JOUR

T1 - Antileishmanial effects of Crotalaria spectabilis Roth aqueous extracts on Leishmania amazonensis

AU - Pacheco, Juliana S.

AU - Teixeira, Érika Maria G. F.

AU - Paschoal, Ramon G.

AU - Torres-Santos, Eduardo Caio

AU - De Simone, Salvatore Giovanni

AU - Da Silva-López, Raquel Elisa

N1 - Funding Information: This study was supported by CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior and Fundação de Apoio para Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E-26/110.139/2009).

PY - 2023

Y1 - 2023

N2 - Fifteen polar extracts from leaf, seed, pod, stem, flower and root of Crotalaria spectabilis were prepared using aqueous systems, based on the principles of green chemistry, and showed different protease inhibitor (PI) activities on trypsin, papain, pepsin and the extracellular L. amazonensis serine protease (LSPIII). The most pronounced inhibitory effect on LSPIII was observed in leaf (CS-P), root, stem, flower (CS-FPVPP) and pod (CS-VA) extracts. Crotalaria extracts exhibited low cytotoxicity on macrophages; however, they decreased the viability of L. amazonensis promastigotes and amastigotes, as observed in leaf (CS-AE, CS-P, CS-T and CS-PVPP), seed (CS-ST), flower and root (CS-RA) extracts. CS-P was chosen to study PI and secondary metabolites and a 10-12 kDa protein, analyzed by mass spectrometry, was identified as a serine PI homologous with papaya latex serine PI. Glycosylated flavonoids, such as quercetins, vitexin and tricin were the major secondary metabolites of CS-P. The presence of PIs in C. spectabilis is a new finding, especially in other organs than seeds since PIs have been reported only in seed legumes. Besides, this is the first report of antileishmanial activity of C. spectabilis extracts and the identification of serine polypeptide PI and glycosylated flavonoids from leaf.

AB - Fifteen polar extracts from leaf, seed, pod, stem, flower and root of Crotalaria spectabilis were prepared using aqueous systems, based on the principles of green chemistry, and showed different protease inhibitor (PI) activities on trypsin, papain, pepsin and the extracellular L. amazonensis serine protease (LSPIII). The most pronounced inhibitory effect on LSPIII was observed in leaf (CS-P), root, stem, flower (CS-FPVPP) and pod (CS-VA) extracts. Crotalaria extracts exhibited low cytotoxicity on macrophages; however, they decreased the viability of L. amazonensis promastigotes and amastigotes, as observed in leaf (CS-AE, CS-P, CS-T and CS-PVPP), seed (CS-ST), flower and root (CS-RA) extracts. CS-P was chosen to study PI and secondary metabolites and a 10-12 kDa protein, analyzed by mass spectrometry, was identified as a serine PI homologous with papaya latex serine PI. Glycosylated flavonoids, such as quercetins, vitexin and tricin were the major secondary metabolites of CS-P. The presence of PIs in C. spectabilis is a new finding, especially in other organs than seeds since PIs have been reported only in seed legumes. Besides, this is the first report of antileishmanial activity of C. spectabilis extracts and the identification of serine polypeptide PI and glycosylated flavonoids from leaf.

KW - Crotalaria

KW - Fabaceae

KW - Serine Proteinase Inhibitors

KW - Antiprotozoal Agents

KW - Flavonoids

KW - Leishmania

KW - Serine

KW - Leishmania pro-teases

KW - aqueous extracts

KW - Crotalaria spectabilis

KW - protease inhibitors

KW - Leishmania viability

KW - flavonoids

UR - https://www.scopus.com/pages/publications/85169999078

U2 - 10.1590/0001-3765202320220613

DO - 10.1590/0001-3765202320220613

M3 - Article

C2 - 37672397

SN - 0001-3765

VL - 95

JO - Anais da Academia Brasileira de Ciências

JF - Anais da Academia Brasileira de Ciências

IS - Supplement 1

M1 - e20220613

ER -