Antisense oligonucleotides made of 2'-O-alkylRNA: their properties and applications in RNA biochemistry

Angus I. Lamond, Brian S. Sproat

Research output: Contribution to journalReview article

89 Citations (Scopus)

Abstract

Oligo(2'-O-alkylribonucleotides) have been developed recently as novel oligonucleotide analogues with properties that enhance their use as antisense probes. They possess high chemical stability and are resistant to hydrolysis by DNA- or RNA-specific nucleases. Many forms of oligo(2'-Oalkylribonucleotides) hybridise specifically and efficiently to complementary RNA sequences, forming stable duplexes that are not substrates for cleavage by RNase H. In combination with prosthetic reporter groups, such as biotin, DNP or fluorophores, oligo(2'-O-alkylribonucleotides) have important applications in a wide range of biochemical studies on RNA function and structure.

Original languageEnglish
Pages (from-to)123-127
Number of pages5
JournalFEBS Letters
Volume325
Issue number1-2
DOIs
Publication statusPublished - 28 Jun 1993

Keywords

  • 2'-O-Alkyl
  • Antisense
  • Oligonucleotide
  • RNA

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